Cytokinin-binding protein from Arabidopsis thaliana leaves participating in transcription regulation

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Cytokinin-binding protein from Arabidopsis thaliana leaves participating in transcription regulation. / Selivankina, S. Y.; Karavaiko, N. N.; Maslova, G. G.; Zubkova, N. K.; Prokoptseva, O. S.; Smith, Aileen R.; Hall, Michael; Kulaeva, O. N.

In: Plant Growth Regulation, Vol. 43, No. 1, 05.2004, p. 15-26.

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Harvard

Selivankina, SY, Karavaiko, NN, Maslova, GG, Zubkova, NK, Prokoptseva, OS, Smith, AR, Hall, M & Kulaeva, ON 2004, 'Cytokinin-binding protein from Arabidopsis thaliana leaves participating in transcription regulation', Plant Growth Regulation, vol. 43, no. 1, pp. 15-26. https://doi.org/10.1023/B:GROW.0000038358.76592.e0

APA

Selivankina, S. Y., Karavaiko, N. N., Maslova, G. G., Zubkova, N. K., Prokoptseva, O. S., Smith, A. R., Hall, M., & Kulaeva, O. N. (2004). Cytokinin-binding protein from Arabidopsis thaliana leaves participating in transcription regulation. Plant Growth Regulation, 43(1), 15-26. https://doi.org/10.1023/B:GROW.0000038358.76592.e0

Vancouver

Selivankina SY, Karavaiko NN, Maslova GG, Zubkova NK, Prokoptseva OS, Smith AR et al. Cytokinin-binding protein from Arabidopsis thaliana leaves participating in transcription regulation. Plant Growth Regulation. 2004 May;43(1):15-26. https://doi.org/10.1023/B:GROW.0000038358.76592.e0

Author

Selivankina, S. Y. ; Karavaiko, N. N. ; Maslova, G. G. ; Zubkova, N. K. ; Prokoptseva, O. S. ; Smith, Aileen R. ; Hall, Michael ; Kulaeva, O. N. / Cytokinin-binding protein from Arabidopsis thaliana leaves participating in transcription regulation. In: Plant Growth Regulation. 2004 ; Vol. 43, No. 1. pp. 15-26.

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@article{06d0d54734b842cf9a6281e26b25fb53,
title = "Cytokinin-binding protein from Arabidopsis thaliana leaves participating in transcription regulation",
abstract = "The 67 kDa cytokinin-binding protein (CBP) has been isolated from old rosette leaves of 9-week-oldArabidopsis thaliana plants. The procedure of CBP isolation included the protein puri?cation by SephadexG-50, hydrophobic chromatography on phenyl-Sepharose and a?nity chromatography on zeatin riboside-Sepharose. Nuclear localization in leaves of 67 kDa CBP was demonstrated. The recognition of a naturalcytokinin trans-zeatin by 67 kDa CBP from Arabidopsis leaves was detected by a very speci?c and sensitiveassay based on trans-zeatin competition in ELISA with anti-idiotype antibodies (Aba-i) for complex formationwith CBP. Aba-i were raised against antibodies against zeatin. trans-Zeatin competed with Aba-i in adose-dependent manner, demonstrating a high a?nity for the protein. In combination with trans-zeatin,67 kDa protein activated RNA synthesis in vitro in the transcription elongation systems containingchromatin-bound RNA polymerase I from barley leaves (heterologous system) or from Arabidopsis leaves(homologous system). These data permit a suggestion that the 67 kDa CBPs from monocots and dicotsful?ll similar functions. A comparison of functional activity of 67 kDa CBP from young, growing rosetteleaves of 3-week-old Arabidopsis plants, from mature leaves of 7-week-old plants and from senescent leavesof 9-week-old plants demonstrated age-dependent changes of the protein properties. The data are discussedin context of recent advances in the study of transcription regulation in eukaryotic cells.",
keywords = "Arabidopsis thaliana, Cytokinin-binding protein, Cytokinins, Receptor, Transcription",
author = "Selivankina, {S. Y.} and Karavaiko, {N. N.} and Maslova, {G. G.} and Zubkova, {N. K.} and Prokoptseva, {O. S.} and Smith, {Aileen R.} and Michael Hall and Kulaeva, {O. N.}",
note = "Selivankina, S. Y., Karavaiko, N. N., Maslova, G. G., Zubkova, N. K., Prokoptseva, O. S., Smith, A. R., Hall, M. A., Kulaeva, O. N. (2004). Cytokinin-binding protein from Arabidopsis thaliana leaves participating in transcription regulation.   Plant Growth Regulation, 43, (1), 15-26 Sponsorship: Russian Foundation for Basic Research, Project (02-04-49367), and by a grant of the President of the Russian Federation for the Support of leading Scientific Schools (NSh-1785.2003.4).",
year = "2004",
month = may,
doi = "10.1023/B:GROW.0000038358.76592.e0",
language = "English",
volume = "43",
pages = "15--26",
journal = "Plant Growth Regulation",
issn = "0167-6903",
publisher = "Springer Nature",
number = "1",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Cytokinin-binding protein from Arabidopsis thaliana leaves participating in transcription regulation

AU - Selivankina, S. Y.

AU - Karavaiko, N. N.

AU - Maslova, G. G.

AU - Zubkova, N. K.

AU - Prokoptseva, O. S.

AU - Smith, Aileen R.

AU - Hall, Michael

AU - Kulaeva, O. N.

N1 - Selivankina, S. Y., Karavaiko, N. N., Maslova, G. G., Zubkova, N. K., Prokoptseva, O. S., Smith, A. R., Hall, M. A., Kulaeva, O. N. (2004). Cytokinin-binding protein from Arabidopsis thaliana leaves participating in transcription regulation.   Plant Growth Regulation, 43, (1), 15-26 Sponsorship: Russian Foundation for Basic Research, Project (02-04-49367), and by a grant of the President of the Russian Federation for the Support of leading Scientific Schools (NSh-1785.2003.4).

PY - 2004/5

Y1 - 2004/5

N2 - The 67 kDa cytokinin-binding protein (CBP) has been isolated from old rosette leaves of 9-week-oldArabidopsis thaliana plants. The procedure of CBP isolation included the protein puri?cation by SephadexG-50, hydrophobic chromatography on phenyl-Sepharose and a?nity chromatography on zeatin riboside-Sepharose. Nuclear localization in leaves of 67 kDa CBP was demonstrated. The recognition of a naturalcytokinin trans-zeatin by 67 kDa CBP from Arabidopsis leaves was detected by a very speci?c and sensitiveassay based on trans-zeatin competition in ELISA with anti-idiotype antibodies (Aba-i) for complex formationwith CBP. Aba-i were raised against antibodies against zeatin. trans-Zeatin competed with Aba-i in adose-dependent manner, demonstrating a high a?nity for the protein. In combination with trans-zeatin,67 kDa protein activated RNA synthesis in vitro in the transcription elongation systems containingchromatin-bound RNA polymerase I from barley leaves (heterologous system) or from Arabidopsis leaves(homologous system). These data permit a suggestion that the 67 kDa CBPs from monocots and dicotsful?ll similar functions. A comparison of functional activity of 67 kDa CBP from young, growing rosetteleaves of 3-week-old Arabidopsis plants, from mature leaves of 7-week-old plants and from senescent leavesof 9-week-old plants demonstrated age-dependent changes of the protein properties. The data are discussedin context of recent advances in the study of transcription regulation in eukaryotic cells.

AB - The 67 kDa cytokinin-binding protein (CBP) has been isolated from old rosette leaves of 9-week-oldArabidopsis thaliana plants. The procedure of CBP isolation included the protein puri?cation by SephadexG-50, hydrophobic chromatography on phenyl-Sepharose and a?nity chromatography on zeatin riboside-Sepharose. Nuclear localization in leaves of 67 kDa CBP was demonstrated. The recognition of a naturalcytokinin trans-zeatin by 67 kDa CBP from Arabidopsis leaves was detected by a very speci?c and sensitiveassay based on trans-zeatin competition in ELISA with anti-idiotype antibodies (Aba-i) for complex formationwith CBP. Aba-i were raised against antibodies against zeatin. trans-Zeatin competed with Aba-i in adose-dependent manner, demonstrating a high a?nity for the protein. In combination with trans-zeatin,67 kDa protein activated RNA synthesis in vitro in the transcription elongation systems containingchromatin-bound RNA polymerase I from barley leaves (heterologous system) or from Arabidopsis leaves(homologous system). These data permit a suggestion that the 67 kDa CBPs from monocots and dicotsful?ll similar functions. A comparison of functional activity of 67 kDa CBP from young, growing rosetteleaves of 3-week-old Arabidopsis plants, from mature leaves of 7-week-old plants and from senescent leavesof 9-week-old plants demonstrated age-dependent changes of the protein properties. The data are discussedin context of recent advances in the study of transcription regulation in eukaryotic cells.

KW - Arabidopsis thaliana

KW - Cytokinin-binding protein

KW - Cytokinins

KW - Receptor

KW - Transcription

U2 - 10.1023/B:GROW.0000038358.76592.e0

DO - 10.1023/B:GROW.0000038358.76592.e0

M3 - Article

VL - 43

SP - 15

EP - 26

JO - Plant Growth Regulation

JF - Plant Growth Regulation

SN - 0167-6903

IS - 1

ER -

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