Comparison of the microbial population in rabbits and guinea pigs by next generation sequencing

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Comparison of the microbial population in rabbits and guinea pigs by next generation sequencing. / Crowley, Edward J.; King, Jonathan J.; Wilkinson, Toby; Worgan, Hilary; Huson, Kathryn Mair; Rose, Michael; McEwan, Neil.

In: PLoS One, Vol. 12, No. 2, e0165779, 09.02.2017.

Research output: Contribution to journalArticlepeer-review

Harvard

Crowley, EJ, King, JJ, Wilkinson, T, Worgan, H, Huson, KM, Rose, M & McEwan, N 2017, 'Comparison of the microbial population in rabbits and guinea pigs by next generation sequencing', PLoS One, vol. 12, no. 2, e0165779. https://doi.org/10.1371/journal.pone.0165779

APA

Crowley, E. J., King, J. J., Wilkinson, T., Worgan, H., Huson, K. M., Rose, M., & McEwan, N. (2017). Comparison of the microbial population in rabbits and guinea pigs by next generation sequencing. PLoS One, 12(2), [e0165779]. https://doi.org/10.1371/journal.pone.0165779

Vancouver

Author

Crowley, Edward J. ; King, Jonathan J. ; Wilkinson, Toby ; Worgan, Hilary ; Huson, Kathryn Mair ; Rose, Michael ; McEwan, Neil. / Comparison of the microbial population in rabbits and guinea pigs by next generation sequencing. In: PLoS One. 2017 ; Vol. 12, No. 2.

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@article{86c6655cc4454f3d837ef147535b01aa,
title = "Comparison of the microbial population in rabbits and guinea pigs by next generation sequencing",
abstract = "This study aimed to determine the microbial composition of faeces from two groups of caecotrophagic animals; rabbits and guinea pigs. In addition the study aimed to determine the community present in the different organs in the rabbit. DNA was extracted from seven of the organs in wild rabbits (n = 5) and from faecal samples from domesticated rabbits (n = 6) and guinea pigs (n = 6). Partial regions of the small ribosomal sub-unit were amplified by PCR and then the sequences present in each sample were determined by next generation sequencing. Differences were detected between samples from rabbit and guinea pig faeces, suggesting that there is not a microbial community common to caecotrophagic animals. Differences were also detected in the different regions of the rabbits{\textquoteright} digestive tracts. As with previous work, many of the organisms detected were Firmicutes or unclassified species and there was a lack of Fibrobacteres, but for the first time we observed a high number of Bacteroidetes in rabbit samples. This work re-iterates high levels of Firmicutes and unclassified species are present in the rabbit gut, together with low number of Fibrobacteres. This suggests that in the rabbit gut, organisms other than the Fibrobacteres must be responsible for fibre digestion. However observation of high numbers of Bacteroidetes suggests that this phylum may indeed have a role to play in digestion in the rabbit gut.",
author = "Crowley, {Edward J.} and King, {Jonathan J.} and Toby Wilkinson and Hilary Worgan and Huson, {Kathryn Mair} and Michael Rose and Neil McEwan",
year = "2017",
month = feb,
day = "9",
doi = "10.1371/journal.pone.0165779",
language = "English",
volume = "12",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "2",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Comparison of the microbial population in rabbits and guinea pigs by next generation sequencing

AU - Crowley, Edward J.

AU - King, Jonathan J.

AU - Wilkinson, Toby

AU - Worgan, Hilary

AU - Huson, Kathryn Mair

AU - Rose, Michael

AU - McEwan, Neil

PY - 2017/2/9

Y1 - 2017/2/9

N2 - This study aimed to determine the microbial composition of faeces from two groups of caecotrophagic animals; rabbits and guinea pigs. In addition the study aimed to determine the community present in the different organs in the rabbit. DNA was extracted from seven of the organs in wild rabbits (n = 5) and from faecal samples from domesticated rabbits (n = 6) and guinea pigs (n = 6). Partial regions of the small ribosomal sub-unit were amplified by PCR and then the sequences present in each sample were determined by next generation sequencing. Differences were detected between samples from rabbit and guinea pig faeces, suggesting that there is not a microbial community common to caecotrophagic animals. Differences were also detected in the different regions of the rabbits’ digestive tracts. As with previous work, many of the organisms detected were Firmicutes or unclassified species and there was a lack of Fibrobacteres, but for the first time we observed a high number of Bacteroidetes in rabbit samples. This work re-iterates high levels of Firmicutes and unclassified species are present in the rabbit gut, together with low number of Fibrobacteres. This suggests that in the rabbit gut, organisms other than the Fibrobacteres must be responsible for fibre digestion. However observation of high numbers of Bacteroidetes suggests that this phylum may indeed have a role to play in digestion in the rabbit gut.

AB - This study aimed to determine the microbial composition of faeces from two groups of caecotrophagic animals; rabbits and guinea pigs. In addition the study aimed to determine the community present in the different organs in the rabbit. DNA was extracted from seven of the organs in wild rabbits (n = 5) and from faecal samples from domesticated rabbits (n = 6) and guinea pigs (n = 6). Partial regions of the small ribosomal sub-unit were amplified by PCR and then the sequences present in each sample were determined by next generation sequencing. Differences were detected between samples from rabbit and guinea pig faeces, suggesting that there is not a microbial community common to caecotrophagic animals. Differences were also detected in the different regions of the rabbits’ digestive tracts. As with previous work, many of the organisms detected were Firmicutes or unclassified species and there was a lack of Fibrobacteres, but for the first time we observed a high number of Bacteroidetes in rabbit samples. This work re-iterates high levels of Firmicutes and unclassified species are present in the rabbit gut, together with low number of Fibrobacteres. This suggests that in the rabbit gut, organisms other than the Fibrobacteres must be responsible for fibre digestion. However observation of high numbers of Bacteroidetes suggests that this phylum may indeed have a role to play in digestion in the rabbit gut.

UR - http://hdl.handle.net/2160/44638

U2 - 10.1371/journal.pone.0165779

DO - 10.1371/journal.pone.0165779

M3 - Article

C2 - 28182658

VL - 12

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 2

M1 - e0165779

ER -

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