Comparison of the microbial population in rabbits and guinea pigs by next generation sequencing
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Comparison of the microbial population in rabbits and guinea pigs by next generation sequencing. / Crowley, Edward J.; King, Jonathan J.; Wilkinson, Toby et al.
In: PLoS One, Vol. 12, No. 2, e0165779, 09.02.2017.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Comparison of the microbial population in rabbits and guinea pigs by next generation sequencing
AU - Crowley, Edward J.
AU - King, Jonathan J.
AU - Wilkinson, Toby
AU - Worgan, Hilary
AU - Huson, Kathryn Mair
AU - Rose, Michael
AU - McEwan, Neil
PY - 2017/2/9
Y1 - 2017/2/9
N2 - This study aimed to determine the microbial composition of faeces from two groups of caecotrophagic animals; rabbits and guinea pigs. In addition the study aimed to determine the community present in the different organs in the rabbit. DNA was extracted from seven of the organs in wild rabbits (n = 5) and from faecal samples from domesticated rabbits (n = 6) and guinea pigs (n = 6). Partial regions of the small ribosomal sub-unit were amplified by PCR and then the sequences present in each sample were determined by next generation sequencing. Differences were detected between samples from rabbit and guinea pig faeces, suggesting that there is not a microbial community common to caecotrophagic animals. Differences were also detected in the different regions of the rabbits’ digestive tracts. As with previous work, many of the organisms detected were Firmicutes or unclassified species and there was a lack of Fibrobacteres, but for the first time we observed a high number of Bacteroidetes in rabbit samples. This work re-iterates high levels of Firmicutes and unclassified species are present in the rabbit gut, together with low number of Fibrobacteres. This suggests that in the rabbit gut, organisms other than the Fibrobacteres must be responsible for fibre digestion. However observation of high numbers of Bacteroidetes suggests that this phylum may indeed have a role to play in digestion in the rabbit gut.
AB - This study aimed to determine the microbial composition of faeces from two groups of caecotrophagic animals; rabbits and guinea pigs. In addition the study aimed to determine the community present in the different organs in the rabbit. DNA was extracted from seven of the organs in wild rabbits (n = 5) and from faecal samples from domesticated rabbits (n = 6) and guinea pigs (n = 6). Partial regions of the small ribosomal sub-unit were amplified by PCR and then the sequences present in each sample were determined by next generation sequencing. Differences were detected between samples from rabbit and guinea pig faeces, suggesting that there is not a microbial community common to caecotrophagic animals. Differences were also detected in the different regions of the rabbits’ digestive tracts. As with previous work, many of the organisms detected were Firmicutes or unclassified species and there was a lack of Fibrobacteres, but for the first time we observed a high number of Bacteroidetes in rabbit samples. This work re-iterates high levels of Firmicutes and unclassified species are present in the rabbit gut, together with low number of Fibrobacteres. This suggests that in the rabbit gut, organisms other than the Fibrobacteres must be responsible for fibre digestion. However observation of high numbers of Bacteroidetes suggests that this phylum may indeed have a role to play in digestion in the rabbit gut.
UR - http://hdl.handle.net/2160/44638
U2 - 10.1371/journal.pone.0165779
DO - 10.1371/journal.pone.0165779
M3 - Article
C2 - 28182658
VL - 12
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 2
M1 - e0165779
ER -