Characterisation of the microbiome along the gastrointestinal tract of growing turkeys

Toby Wilkinson; Alexander Cowan; Hannah Vallin; L. A. Onime; Linda Boniface Oyama; Simon Cameron; Charlotte Gonot; Jonathan Moorby; Kate Waddams; Vincent Theobald; David Leemans; S. Bowra; C. Nixey; Sharon Huws

doi:10.3389/fmicb.2017.01089

Characterisation of the microbiome along the gastrointestinal tract of growing turkeys

Standard

Characterisation of the microbiome along the gastrointestinal tract of growing turkeys. / Wilkinson, Toby; Cowan, Alexander ; Vallin, Hannah; Onime, L. A.; Oyama, Linda Boniface; Cameron, Simon; Gonot, Charlotte; Moorby, Jonathan; Waddams, Kate; Theobald, Vincent; Leemans, David; Bowra, S.; Nixey, C.; Huws, Sharon.

In: Frontiers in Microbiology, Vol. 8, 1089, 22.06.2017.

Research output: Contribution to journal › Article › peer-review

Harvard

Wilkinson, T, Cowan, A , Vallin, H, Onime, LA, Oyama, LB, Cameron, S, Gonot, C, Moorby, J, Waddams, K, Theobald, V, Leemans, D, Bowra, S, Nixey, C & Huws, S 2017, 'Characterisation of the microbiome along the gastrointestinal tract of growing turkeys', Frontiers in Microbiology, vol. 8, 1089. https://doi.org/10.3389/fmicb.2017.01089

APA

Wilkinson, T., Cowan, A., Vallin, H., Onime, L. A., Oyama, L. B., Cameron, S., Gonot, C., Moorby, J., Waddams, K., Theobald, V., Leemans, D., Bowra, S., Nixey, C., & Huws, S. (2017). Characterisation of the microbiome along the gastrointestinal tract of growing turkeys. Frontiers in Microbiology, 8, [1089]. https://doi.org/10.3389/fmicb.2017.01089

Vancouver

Wilkinson T, Cowan A , Vallin H, Onime LA, Oyama LB, Cameron S et al. Characterisation of the microbiome along the gastrointestinal tract of growing turkeys. Frontiers in Microbiology. 2017 Jun 22;8. 1089. https://doi.org/10.3389/fmicb.2017.01089

Author

Wilkinson, Toby ; Cowan, Alexander ; Vallin, Hannah ; Onime, L. A. ; Oyama, Linda Boniface ; Cameron, Simon ; Gonot, Charlotte ; Moorby, Jonathan ; Waddams, Kate ; Theobald, Vincent ; Leemans, David ; Bowra, S. ; Nixey, C. ; Huws, Sharon. / Characterisation of the microbiome along the gastrointestinal tract of growing turkeys. In: Frontiers in Microbiology. 2017 ; Vol. 8.

Bibtex - Download

@article{4d237a169cf945c1aff45e8610f1c71b,

title = "Characterisation of the microbiome along the gastrointestinal tract of growing turkeys",

abstract = "The turkey microbiome is largely understudied, despite its relationship with bird health and growth, and the prevalence of human pathogens such as Campylobacter spp. In this study we investigated the microbiome within the small intestine (SI), caeca (C), large intestine (LI) and cloaca (CL) of turkeys at 6, 10 and 16 weeks of age. Eight turkeys were dissected within each age category and the contents of the SI, C, LI and CL were harvested. 16S rDNA based QPCR was performed on all samples and samples for the 4 locations within 3 birds/age group were sequenced using ion torrent-based sequencing of the 16S rDNA. Sequencing data showed on a genus level, an abundance of Lactobacillus, Streptococcus and Clostridium XI (38.2, 28.1 and 13.0% respectively) irrespective of location and age. The caeca exhibited the greatest microbiome diversity throughout the development of the turkey. PICRUSt data predicted an array of bacterial function, with most differences being apparent in the caeca of the turkeys as they matured. QPCR revealed that the caeca within 10 week old birds, contained the most Campylobacter spp. Understanding the microbial ecology of the turkey gastrointestinal tract is essential in terms of understanding production efficiency and in order to develop novel strategies for targeting Campylobacter spp",

keywords = "turkey, 16S rDNA, microbiome, Campylobacter, gastrointestinal tract, small intestine, casecum, large intestine",

author = "Toby Wilkinson and Alexander Cowan and Hannah Vallin and Onime, {L. A.} and Oyama, {Linda Boniface} and Simon Cameron and Charlotte Gonot and Jonathan Moorby and Kate Waddams and Vincent Theobald and David Leemans and S. Bowra and C. Nixey and Sharon Huws",

year = "2017",

month = jun,

day = "22",

doi = "10.3389/fmicb.2017.01089",

language = "English",

volume = "8",

journal = "Frontiers in Microbiology",

issn = "1664-302X",

publisher = "Frontiers Media",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Characterisation of the microbiome along the gastrointestinal tract of growing turkeys

AU - Wilkinson, Toby

AU - Cowan, Alexander

AU - Vallin, Hannah

AU - Onime, L. A.

AU - Oyama, Linda Boniface

AU - Cameron, Simon

AU - Gonot, Charlotte

AU - Moorby, Jonathan

AU - Waddams, Kate

AU - Theobald, Vincent

AU - Leemans, David

AU - Bowra, S.

AU - Nixey, C.

AU - Huws, Sharon

PY - 2017/6/22

Y1 - 2017/6/22

N2 - The turkey microbiome is largely understudied, despite its relationship with bird health and growth, and the prevalence of human pathogens such as Campylobacter spp. In this study we investigated the microbiome within the small intestine (SI), caeca (C), large intestine (LI) and cloaca (CL) of turkeys at 6, 10 and 16 weeks of age. Eight turkeys were dissected within each age category and the contents of the SI, C, LI and CL were harvested. 16S rDNA based QPCR was performed on all samples and samples for the 4 locations within 3 birds/age group were sequenced using ion torrent-based sequencing of the 16S rDNA. Sequencing data showed on a genus level, an abundance of Lactobacillus, Streptococcus and Clostridium XI (38.2, 28.1 and 13.0% respectively) irrespective of location and age. The caeca exhibited the greatest microbiome diversity throughout the development of the turkey. PICRUSt data predicted an array of bacterial function, with most differences being apparent in the caeca of the turkeys as they matured. QPCR revealed that the caeca within 10 week old birds, contained the most Campylobacter spp. Understanding the microbial ecology of the turkey gastrointestinal tract is essential in terms of understanding production efficiency and in order to develop novel strategies for targeting Campylobacter spp

AB - The turkey microbiome is largely understudied, despite its relationship with bird health and growth, and the prevalence of human pathogens such as Campylobacter spp. In this study we investigated the microbiome within the small intestine (SI), caeca (C), large intestine (LI) and cloaca (CL) of turkeys at 6, 10 and 16 weeks of age. Eight turkeys were dissected within each age category and the contents of the SI, C, LI and CL were harvested. 16S rDNA based QPCR was performed on all samples and samples for the 4 locations within 3 birds/age group were sequenced using ion torrent-based sequencing of the 16S rDNA. Sequencing data showed on a genus level, an abundance of Lactobacillus, Streptococcus and Clostridium XI (38.2, 28.1 and 13.0% respectively) irrespective of location and age. The caeca exhibited the greatest microbiome diversity throughout the development of the turkey. PICRUSt data predicted an array of bacterial function, with most differences being apparent in the caeca of the turkeys as they matured. QPCR revealed that the caeca within 10 week old birds, contained the most Campylobacter spp. Understanding the microbial ecology of the turkey gastrointestinal tract is essential in terms of understanding production efficiency and in order to develop novel strategies for targeting Campylobacter spp

KW - turkey

KW - 16S rDNA

KW - microbiome

KW - Campylobacter

KW - gastrointestinal tract

KW - small intestine

KW - casecum

KW - large intestine

UR - http://hdl.handle.net/2160/45307

U2 - 10.3389/fmicb.2017.01089

DO - 10.3389/fmicb.2017.01089

M3 - Article