Cell-Type Specific Roles for PTEN in Establishing a Functional Retinal Architecture

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Cell-Type Specific Roles for PTEN in Establishing a Functional Retinal Architecture. / Cantrup, Robert; Dixit, Rajiv; Palmesino, Elena; Bonfield, Stephan; Shaker, Tarek; Tachibana, Nobuhiko; Zinyk, Dawn; Dalesman, Sarah; Yamakawa, Kazuhiro; Stell, William K.; Wong, Rachel O.; Reese, Benjamin E.; Kania, Artur; Sauvé, Yves; Schuurmans, Carol.

In: PLoS One, Vol. 7, No. 3, e32795, 05.03.2012.

Research output: Contribution to journalArticle

Harvard

Cantrup, R, Dixit, R, Palmesino, E, Bonfield, S, Shaker, T, Tachibana, N, Zinyk, D, Dalesman, S, Yamakawa, K, Stell, WK, Wong, RO, Reese, BE, Kania, A, Sauvé, Y & Schuurmans, C 2012, 'Cell-Type Specific Roles for PTEN in Establishing a Functional Retinal Architecture' PLoS One, vol. 7, no. 3, e32795. https://doi.org/10.1371/journal.pone.0032795

APA

Cantrup, R., Dixit, R., Palmesino, E., Bonfield, S., Shaker, T., Tachibana, N., ... Schuurmans, C. (2012). Cell-Type Specific Roles for PTEN in Establishing a Functional Retinal Architecture. PLoS One, 7(3), [e32795]. https://doi.org/10.1371/journal.pone.0032795

Vancouver

Cantrup R, Dixit R, Palmesino E, Bonfield S, Shaker T, Tachibana N et al. Cell-Type Specific Roles for PTEN in Establishing a Functional Retinal Architecture. PLoS One. 2012 Mar 5;7(3). e32795. https://doi.org/10.1371/journal.pone.0032795

Author

Cantrup, Robert ; Dixit, Rajiv ; Palmesino, Elena ; Bonfield, Stephan ; Shaker, Tarek ; Tachibana, Nobuhiko ; Zinyk, Dawn ; Dalesman, Sarah ; Yamakawa, Kazuhiro ; Stell, William K. ; Wong, Rachel O. ; Reese, Benjamin E. ; Kania, Artur ; Sauvé, Yves ; Schuurmans, Carol. / Cell-Type Specific Roles for PTEN in Establishing a Functional Retinal Architecture. In: PLoS One. 2012 ; Vol. 7, No. 3.

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@article{bfe7f3c8e8ca4f9ca9dcf0b9efed8dbd,
title = "Cell-Type Specific Roles for PTEN in Establishing a Functional Retinal Architecture",
abstract = "BackgroundThe retina has a unique three-dimensional architecture, the precise organization of which allows for complete sampling of the visual field. Along the radial or apicobasal axis, retinal neurons and their dendritic and axonal arbors are segregated into layers, while perpendicular to this axis, in the tangential plane, four of the six neuronal types form patterned cellular arrays, or mosaics. Currently, the molecular cues that control retinal cell positioning are not well-understood, especially those that operate in the tangential plane. Here we investigated the role of the PTEN phosphatase in establishing a functional retinal architecture.Methodology/Principal FindingsIn the developing retina, PTEN was localized preferentially to ganglion, amacrine and horizontal cells, whose somata are distributed in mosaic patterns in the tangential plane. Generation of a retina-specific Pten knock-out resulted in retinal ganglion, amacrine and horizontal cell hypertrophy, and expansion of the inner plexiform layer. The spacing of Pten mutant mosaic populations was also aberrant, as were the arborization and fasciculation patterns of their processes, displaying cell type-specific defects in the radial and tangential dimensions. Irregular oscillatory potentials were also observed in Pten mutant electroretinograms, indicative of asynchronous amacrine cell firing. Furthermore, while Pten mutant RGC axons targeted appropriate brain regions, optokinetic spatial acuity was reduced in Pten mutant animals. Finally, while some features of the Pten mutant retina appeared similar to those reported in Dscam-mutant mice, PTEN expression and activity were normal in the absence of Dscam.Conclusions/SignificanceWe conclude that Pten regulates somal positioning and neurite arborization patterns of a subset of retinal cells that form mosaics, likely functioning independently of Dscam, at least during the embryonic period. Our findings thus reveal an unexpected level of cellular specificity for the multi-purpose phosphatase, and identify Pten as an integral component of a novel cell positioning pathway in the retina.",
author = "Robert Cantrup and Rajiv Dixit and Elena Palmesino and Stephan Bonfield and Tarek Shaker and Nobuhiko Tachibana and Dawn Zinyk and Sarah Dalesman and Kazuhiro Yamakawa and Stell, {William K.} and Wong, {Rachel O.} and Reese, {Benjamin E.} and Artur Kania and Yves Sauv{\'e} and Carol Schuurmans",
note = "Cantrup, R., Dixit, R., Palmesino, E., Bonfield, S., Shaker, T., Tachibana, N., Zinyk, D., Dalesman, S., Yamakawa, K., Stell, W. K., Wong, R. O., Reese, B. E., Kania, A., Sauv{\'e}, Y. & Schuurmans, C. (2012). Cell-type specific roles for pten in establishing a functional retinal architecture. PLoS One, 7 (3), [e32795]",
year = "2012",
month = "3",
day = "5",
doi = "10.1371/journal.pone.0032795",
language = "English",
volume = "7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Cell-Type Specific Roles for PTEN in Establishing a Functional Retinal Architecture

AU - Cantrup, Robert

AU - Dixit, Rajiv

AU - Palmesino, Elena

AU - Bonfield, Stephan

AU - Shaker, Tarek

AU - Tachibana, Nobuhiko

AU - Zinyk, Dawn

AU - Dalesman, Sarah

AU - Yamakawa, Kazuhiro

AU - Stell, William K.

AU - Wong, Rachel O.

AU - Reese, Benjamin E.

AU - Kania, Artur

AU - Sauvé, Yves

AU - Schuurmans, Carol

N1 - Cantrup, R., Dixit, R., Palmesino, E., Bonfield, S., Shaker, T., Tachibana, N., Zinyk, D., Dalesman, S., Yamakawa, K., Stell, W. K., Wong, R. O., Reese, B. E., Kania, A., Sauvé, Y. & Schuurmans, C. (2012). Cell-type specific roles for pten in establishing a functional retinal architecture. PLoS One, 7 (3), [e32795]

PY - 2012/3/5

Y1 - 2012/3/5

N2 - BackgroundThe retina has a unique three-dimensional architecture, the precise organization of which allows for complete sampling of the visual field. Along the radial or apicobasal axis, retinal neurons and their dendritic and axonal arbors are segregated into layers, while perpendicular to this axis, in the tangential plane, four of the six neuronal types form patterned cellular arrays, or mosaics. Currently, the molecular cues that control retinal cell positioning are not well-understood, especially those that operate in the tangential plane. Here we investigated the role of the PTEN phosphatase in establishing a functional retinal architecture.Methodology/Principal FindingsIn the developing retina, PTEN was localized preferentially to ganglion, amacrine and horizontal cells, whose somata are distributed in mosaic patterns in the tangential plane. Generation of a retina-specific Pten knock-out resulted in retinal ganglion, amacrine and horizontal cell hypertrophy, and expansion of the inner plexiform layer. The spacing of Pten mutant mosaic populations was also aberrant, as were the arborization and fasciculation patterns of their processes, displaying cell type-specific defects in the radial and tangential dimensions. Irregular oscillatory potentials were also observed in Pten mutant electroretinograms, indicative of asynchronous amacrine cell firing. Furthermore, while Pten mutant RGC axons targeted appropriate brain regions, optokinetic spatial acuity was reduced in Pten mutant animals. Finally, while some features of the Pten mutant retina appeared similar to those reported in Dscam-mutant mice, PTEN expression and activity were normal in the absence of Dscam.Conclusions/SignificanceWe conclude that Pten regulates somal positioning and neurite arborization patterns of a subset of retinal cells that form mosaics, likely functioning independently of Dscam, at least during the embryonic period. Our findings thus reveal an unexpected level of cellular specificity for the multi-purpose phosphatase, and identify Pten as an integral component of a novel cell positioning pathway in the retina.

AB - BackgroundThe retina has a unique three-dimensional architecture, the precise organization of which allows for complete sampling of the visual field. Along the radial or apicobasal axis, retinal neurons and their dendritic and axonal arbors are segregated into layers, while perpendicular to this axis, in the tangential plane, four of the six neuronal types form patterned cellular arrays, or mosaics. Currently, the molecular cues that control retinal cell positioning are not well-understood, especially those that operate in the tangential plane. Here we investigated the role of the PTEN phosphatase in establishing a functional retinal architecture.Methodology/Principal FindingsIn the developing retina, PTEN was localized preferentially to ganglion, amacrine and horizontal cells, whose somata are distributed in mosaic patterns in the tangential plane. Generation of a retina-specific Pten knock-out resulted in retinal ganglion, amacrine and horizontal cell hypertrophy, and expansion of the inner plexiform layer. The spacing of Pten mutant mosaic populations was also aberrant, as were the arborization and fasciculation patterns of their processes, displaying cell type-specific defects in the radial and tangential dimensions. Irregular oscillatory potentials were also observed in Pten mutant electroretinograms, indicative of asynchronous amacrine cell firing. Furthermore, while Pten mutant RGC axons targeted appropriate brain regions, optokinetic spatial acuity was reduced in Pten mutant animals. Finally, while some features of the Pten mutant retina appeared similar to those reported in Dscam-mutant mice, PTEN expression and activity were normal in the absence of Dscam.Conclusions/SignificanceWe conclude that Pten regulates somal positioning and neurite arborization patterns of a subset of retinal cells that form mosaics, likely functioning independently of Dscam, at least during the embryonic period. Our findings thus reveal an unexpected level of cellular specificity for the multi-purpose phosphatase, and identify Pten as an integral component of a novel cell positioning pathway in the retina.

UR - http://www.scopus.com/inward/record.url?scp=84857748372&partnerID=8YFLogxK

UR - http://hdl.handle.net/2160/26369

U2 - 10.1371/journal.pone.0032795

DO - 10.1371/journal.pone.0032795

M3 - Article

VL - 7

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 3

M1 - e32795

ER -

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