Breeding for Bio-ethanol Production in Lolium perenne L.Association of Allelic Variation with High Water-Soluble Carbohydrate Content

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Breeding for Bio-ethanol Production in Lolium perenne L. Association of Allelic Variation with High Water-Soluble Carbohydrate Content. / Farrar, Kerrie; Bryant, David Neil; Turner, Lesley Beryl; Gallagher, Joseph Anthony; Thomas, Ann Myfanwy; Farrell, Markku Sefton; Humphreys, Mervyn Owen; Donnison, Iain Simon.

In: Bioenergy Research, Vol. 5, No. 1, 03.2012, p. 149-157.

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@article{9971927c4f064803be81b933c33fc111,
title = "Breeding for Bio-ethanol Production in Lolium perenne L.: Association of Allelic Variation with High Water-Soluble Carbohydrate Content",
abstract = "Increasing the extractable sugar yield from perennial crops is one strategy to generate renewable fuels such as bio-ethanol. Lolium perenne L. (perennial ryegrass) can contain significant (>30% dry matter) water-soluble sugars in the form of polymeric fructan which is readily extracted, broken down and fermented to bioethanol. A population of Lolium perenne generated from four parents which differed in water-soluble carbohydrate (WSC) content was subjected to multiple rounds of selection and recombination on the basis of early spring WSC content to produce a high WSC and a low WSC population. A control population was generated by selecting the same number of plants at random. The alleles present at six candidate gene loci were analysed before and after selection and correlated to WSC content. Significant differences in the allele frequency of L. perenne soluble-acid invertase1:4 were observed between the three populations with one haplotype significantly associated with the high WSC C2(S+) population (after three rounds of selection and two rounds of recombination). Moreover, WSC content was also associated with biomass accumulation. Thus, in addition to a 2.84-fold increase in WSC yield, the C2(S+) population also had 1.48-fold more biomass per plant, resulting in 3.9-fold higher WSC yield per plant than the control population.",
keywords = "Single-nucleotide polymorphisms (SNPs), Marker-assisted selection (MAS), Phenomics, Single-strand conformational polymorphism (SSCP), Invertase1:4 (LpsaINV1:4)",
author = "Kerrie Farrar and Bryant, {David Neil} and Turner, {Lesley Beryl} and Gallagher, {Joseph Anthony} and Thomas, {Ann Myfanwy} and Farrell, {Markku Sefton} and Humphreys, {Mervyn Owen} and Donnison, {Iain Simon}",
note = "Sponsorship: BBSRC RONO: BBS/E/W/00003134B; BB/E024319/2; 03134 CFC ",
year = "2012",
month = mar,
doi = "10.1007/s12155-011-9156-0",
language = "English",
volume = "5",
pages = "149--157",
journal = "Bioenergy Research",
issn = "1939-1234",
publisher = "Springer Nature",
number = "1",

}

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TY - JOUR

T1 - Breeding for Bio-ethanol Production in Lolium perenne L.

T2 - Association of Allelic Variation with High Water-Soluble Carbohydrate Content

AU - Farrar, Kerrie

AU - Bryant, David Neil

AU - Turner, Lesley Beryl

AU - Gallagher, Joseph Anthony

AU - Thomas, Ann Myfanwy

AU - Farrell, Markku Sefton

AU - Humphreys, Mervyn Owen

AU - Donnison, Iain Simon

N1 - Sponsorship: BBSRC RONO: BBS/E/W/00003134B; BB/E024319/2; 03134 CFC

PY - 2012/3

Y1 - 2012/3

N2 - Increasing the extractable sugar yield from perennial crops is one strategy to generate renewable fuels such as bio-ethanol. Lolium perenne L. (perennial ryegrass) can contain significant (>30% dry matter) water-soluble sugars in the form of polymeric fructan which is readily extracted, broken down and fermented to bioethanol. A population of Lolium perenne generated from four parents which differed in water-soluble carbohydrate (WSC) content was subjected to multiple rounds of selection and recombination on the basis of early spring WSC content to produce a high WSC and a low WSC population. A control population was generated by selecting the same number of plants at random. The alleles present at six candidate gene loci were analysed before and after selection and correlated to WSC content. Significant differences in the allele frequency of L. perenne soluble-acid invertase1:4 were observed between the three populations with one haplotype significantly associated with the high WSC C2(S+) population (after three rounds of selection and two rounds of recombination). Moreover, WSC content was also associated with biomass accumulation. Thus, in addition to a 2.84-fold increase in WSC yield, the C2(S+) population also had 1.48-fold more biomass per plant, resulting in 3.9-fold higher WSC yield per plant than the control population.

AB - Increasing the extractable sugar yield from perennial crops is one strategy to generate renewable fuels such as bio-ethanol. Lolium perenne L. (perennial ryegrass) can contain significant (>30% dry matter) water-soluble sugars in the form of polymeric fructan which is readily extracted, broken down and fermented to bioethanol. A population of Lolium perenne generated from four parents which differed in water-soluble carbohydrate (WSC) content was subjected to multiple rounds of selection and recombination on the basis of early spring WSC content to produce a high WSC and a low WSC population. A control population was generated by selecting the same number of plants at random. The alleles present at six candidate gene loci were analysed before and after selection and correlated to WSC content. Significant differences in the allele frequency of L. perenne soluble-acid invertase1:4 were observed between the three populations with one haplotype significantly associated with the high WSC C2(S+) population (after three rounds of selection and two rounds of recombination). Moreover, WSC content was also associated with biomass accumulation. Thus, in addition to a 2.84-fold increase in WSC yield, the C2(S+) population also had 1.48-fold more biomass per plant, resulting in 3.9-fold higher WSC yield per plant than the control population.

KW - Single-nucleotide polymorphisms (SNPs)

KW - Marker-assisted selection (MAS)

KW - Phenomics

KW - Single-strand conformational polymorphism (SSCP)

KW - Invertase1:4 (LpsaINV1:4)

UR - http://hdl.handle.net/2160/8118

U2 - 10.1007/s12155-011-9156-0

DO - 10.1007/s12155-011-9156-0

M3 - Article

C2 - 26366245

VL - 5

SP - 149

EP - 157

JO - Bioenergy Research

JF - Bioenergy Research

SN - 1939-1234

IS - 1

ER -

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