Biorefining Potential of Wild-Grown Arundo donax, Cortaderia selloana and Phragmites australis and the Feasibility of White-Rot Fungi-Mediated Pretreatments

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Biorefining Potential of Wild-Grown Arundo donax, Cortaderia selloana and Phragmites australis and the Feasibility of White-Rot Fungi-Mediated Pretreatments. / da Costa, Ricardo M.F.; Winters, Ana; Hauck, Barbara; Martín, Daniel; Bosch, Maurice; Simister, Rachael; Gomez, Leonardo D.; Batista de Carvalho, Luís A.E.; Canhoto, Jorge M.

In: Frontiers in Plant Science, Vol. 12, 679966, 02.07.2021.

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da Costa, R. M. F., Winters, A., Hauck, B., Martín, D., Bosch, M., Simister, R., Gomez, L. D., Batista de Carvalho, L. A. E., & Canhoto, J. M. (2021). Biorefining Potential of Wild-Grown Arundo donax, Cortaderia selloana and Phragmites australis and the Feasibility of White-Rot Fungi-Mediated Pretreatments. Frontiers in Plant Science, 12, [679966]. https://doi.org/10.3389/fpls.2021.679966

Author

da Costa, Ricardo M.F. ; Winters, Ana ; Hauck, Barbara ; Martín, Daniel ; Bosch, Maurice ; Simister, Rachael ; Gomez, Leonardo D. ; Batista de Carvalho, Luís A.E. ; Canhoto, Jorge M. / Biorefining Potential of Wild-Grown Arundo donax, Cortaderia selloana and Phragmites australis and the Feasibility of White-Rot Fungi-Mediated Pretreatments. In: Frontiers in Plant Science. 2021 ; Vol. 12.

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@article{94c5030996914355aba9705979b27edd,
title = "Biorefining Potential of Wild-Grown Arundo donax, Cortaderia selloana and Phragmites australis and the Feasibility of White-Rot Fungi-Mediated Pretreatments",
abstract = "Arundo donax, Cortaderia selloana and Phragmites australis are high-biomass-producing perennial Poalean species that grow abundantly and spontaneously in warm temperate regions, such as in Mediterranean-type climates, like those of Southern Europe, Western United States coastal areas, or in regions of South America, South Africa and Australia. Given their vigorous and spontaneous growth, biomass from the studied grasses often accumulates excessively in unmanaged agro-forestry areas. Nonetheless, this also creates the demand and opportunity for the valorisation of these biomass sources, particularly their cell wall polymers, for biorefining applications. By contrast, a related crop, Miscanthus × giganteus, is a perennial grass that has been extensively studied for lignocellulosic biomass production, as it can grow on low-input agricultural systems in colder climates. In this study Fourier transform mid-infrared spectroscopy (FTIR), high-performance anion-exchange chromatography (HPAEC) and lignin content determinations were used for a comparative compositional characterisation of A. donax, C. selloana and P. australis harvested from the wild, in relation to a trial field-grown M. × giganteus high-yielding genotype. A high-throughput saccharification assay showed relatively high sugar release values from the wild-grown grasses, even with a 0.1M NaOH mild alkali pretreatment. In addition to this alkaline pretreatment, biomass was treated with white-rot fungi (WRF), which preferentially degrade lignin more readily than holocellulose. Three fungal species were used: Ganoderma lucidum, Pleurotus ostreatus and Trametes versicolor. Our results showed that neutral sugar contents are not significantly altered, while some lignin is lost during the pretreatments. Furthermore, sugar release upon enzymatic saccharification was enhanced, and this was dependent on the plant biomass and fungal species used in the treatment. To maximise the potential for lignocellulose valorisation, the liquid fractions from the pretreatments were analysed by high performance liquid chromatography – photodiode array detection – electrospray ionisation tandem mass spectrometry (HPLC-PDA-ESI-MSn). This study is one of the first to report on the composition of WRF-treated grass biomass, while assessing the potential relevance of breakdown products released during the treatments, beyond more traditional sugar-for-energy applications. Ultimately, we expect that our data will help promote the valorisation of unused biomass resources, create economic value, while contributing to the implementation of sustainable biorefining systems.",
keywords = "Arundo donax, biomass, cell wall, Cortaderia selloana, marginal lands, Miscanthus × giganteus, Phragmites australis, white-rot fungi",
author = "{da Costa}, {Ricardo M.F.} and Ana Winters and Barbara Hauck and Daniel Mart{\'i}n and Maurice Bosch and Rachael Simister and Gomez, {Leonardo D.} and {Batista de Carvalho}, {Lu{\'i}s A.E.} and Canhoto, {Jorge M.}",
note = "Funding Information: We would like to express our gratitude to the Centre for Novel Agricultural Products (CNAP) of University of York for generously allowing the use of high throughput saccharification assay instrumentation available at their laboratories. Funding. This work was supported by the Project ?RENATURE ? Valorisation of the Natural Endogenous Resources of the Centro Region? (CENTRO-01-0145-FEDER-000007), co-financed by the Comiss?o de Coordena??o da Regi?o Centro (CCDR-C) and by the European Regional Developmental Fund (ERDF) and by Portuguese Foundation for Science and Technology (FCT) (UIDB/00070/2020). Further support came from the BBSRC Core Strategic Programme in Resilient Crops: Miscanthus (BBS/E/W/0012843A) and from a Newton Fund RCUK-CONFAP Research Partnership (BBSRC grant number BB/M029212/1). We acknowledge that this study is partly based upon work performed during a Short-Term Scientific Mission (STSM) supported by COST (European Cooperation in Science and Technology), via COST Action CA17128 ? ?Establishment of a Pan-European Network on the Sustainable Valorisation of Lignin? (https://lignocost.eu/). Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 da Costa, Winters, Hauck, Mart{\'i}n, Bosch, Simister, Gomez, Batista de Carvalho and Canhoto.",
year = "2021",
month = jul,
day = "2",
doi = "10.3389/fpls.2021.679966",
language = "English",
volume = "12",
journal = "Frontiers in Plant Science",
issn = "1664-462X",
publisher = "Frontiers Media",

}

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TY - JOUR

T1 - Biorefining Potential of Wild-Grown Arundo donax, Cortaderia selloana and Phragmites australis and the Feasibility of White-Rot Fungi-Mediated Pretreatments

AU - da Costa, Ricardo M.F.

AU - Winters, Ana

AU - Hauck, Barbara

AU - Martín, Daniel

AU - Bosch, Maurice

AU - Simister, Rachael

AU - Gomez, Leonardo D.

AU - Batista de Carvalho, Luís A.E.

AU - Canhoto, Jorge M.

N1 - Funding Information: We would like to express our gratitude to the Centre for Novel Agricultural Products (CNAP) of University of York for generously allowing the use of high throughput saccharification assay instrumentation available at their laboratories. Funding. This work was supported by the Project ?RENATURE ? Valorisation of the Natural Endogenous Resources of the Centro Region? (CENTRO-01-0145-FEDER-000007), co-financed by the Comiss?o de Coordena??o da Regi?o Centro (CCDR-C) and by the European Regional Developmental Fund (ERDF) and by Portuguese Foundation for Science and Technology (FCT) (UIDB/00070/2020). Further support came from the BBSRC Core Strategic Programme in Resilient Crops: Miscanthus (BBS/E/W/0012843A) and from a Newton Fund RCUK-CONFAP Research Partnership (BBSRC grant number BB/M029212/1). We acknowledge that this study is partly based upon work performed during a Short-Term Scientific Mission (STSM) supported by COST (European Cooperation in Science and Technology), via COST Action CA17128 ? ?Establishment of a Pan-European Network on the Sustainable Valorisation of Lignin? (https://lignocost.eu/). Publisher Copyright: © Copyright © 2021 da Costa, Winters, Hauck, Martín, Bosch, Simister, Gomez, Batista de Carvalho and Canhoto.

PY - 2021/7/2

Y1 - 2021/7/2

N2 - Arundo donax, Cortaderia selloana and Phragmites australis are high-biomass-producing perennial Poalean species that grow abundantly and spontaneously in warm temperate regions, such as in Mediterranean-type climates, like those of Southern Europe, Western United States coastal areas, or in regions of South America, South Africa and Australia. Given their vigorous and spontaneous growth, biomass from the studied grasses often accumulates excessively in unmanaged agro-forestry areas. Nonetheless, this also creates the demand and opportunity for the valorisation of these biomass sources, particularly their cell wall polymers, for biorefining applications. By contrast, a related crop, Miscanthus × giganteus, is a perennial grass that has been extensively studied for lignocellulosic biomass production, as it can grow on low-input agricultural systems in colder climates. In this study Fourier transform mid-infrared spectroscopy (FTIR), high-performance anion-exchange chromatography (HPAEC) and lignin content determinations were used for a comparative compositional characterisation of A. donax, C. selloana and P. australis harvested from the wild, in relation to a trial field-grown M. × giganteus high-yielding genotype. A high-throughput saccharification assay showed relatively high sugar release values from the wild-grown grasses, even with a 0.1M NaOH mild alkali pretreatment. In addition to this alkaline pretreatment, biomass was treated with white-rot fungi (WRF), which preferentially degrade lignin more readily than holocellulose. Three fungal species were used: Ganoderma lucidum, Pleurotus ostreatus and Trametes versicolor. Our results showed that neutral sugar contents are not significantly altered, while some lignin is lost during the pretreatments. Furthermore, sugar release upon enzymatic saccharification was enhanced, and this was dependent on the plant biomass and fungal species used in the treatment. To maximise the potential for lignocellulose valorisation, the liquid fractions from the pretreatments were analysed by high performance liquid chromatography – photodiode array detection – electrospray ionisation tandem mass spectrometry (HPLC-PDA-ESI-MSn). This study is one of the first to report on the composition of WRF-treated grass biomass, while assessing the potential relevance of breakdown products released during the treatments, beyond more traditional sugar-for-energy applications. Ultimately, we expect that our data will help promote the valorisation of unused biomass resources, create economic value, while contributing to the implementation of sustainable biorefining systems.

AB - Arundo donax, Cortaderia selloana and Phragmites australis are high-biomass-producing perennial Poalean species that grow abundantly and spontaneously in warm temperate regions, such as in Mediterranean-type climates, like those of Southern Europe, Western United States coastal areas, or in regions of South America, South Africa and Australia. Given their vigorous and spontaneous growth, biomass from the studied grasses often accumulates excessively in unmanaged agro-forestry areas. Nonetheless, this also creates the demand and opportunity for the valorisation of these biomass sources, particularly their cell wall polymers, for biorefining applications. By contrast, a related crop, Miscanthus × giganteus, is a perennial grass that has been extensively studied for lignocellulosic biomass production, as it can grow on low-input agricultural systems in colder climates. In this study Fourier transform mid-infrared spectroscopy (FTIR), high-performance anion-exchange chromatography (HPAEC) and lignin content determinations were used for a comparative compositional characterisation of A. donax, C. selloana and P. australis harvested from the wild, in relation to a trial field-grown M. × giganteus high-yielding genotype. A high-throughput saccharification assay showed relatively high sugar release values from the wild-grown grasses, even with a 0.1M NaOH mild alkali pretreatment. In addition to this alkaline pretreatment, biomass was treated with white-rot fungi (WRF), which preferentially degrade lignin more readily than holocellulose. Three fungal species were used: Ganoderma lucidum, Pleurotus ostreatus and Trametes versicolor. Our results showed that neutral sugar contents are not significantly altered, while some lignin is lost during the pretreatments. Furthermore, sugar release upon enzymatic saccharification was enhanced, and this was dependent on the plant biomass and fungal species used in the treatment. To maximise the potential for lignocellulose valorisation, the liquid fractions from the pretreatments were analysed by high performance liquid chromatography – photodiode array detection – electrospray ionisation tandem mass spectrometry (HPLC-PDA-ESI-MSn). This study is one of the first to report on the composition of WRF-treated grass biomass, while assessing the potential relevance of breakdown products released during the treatments, beyond more traditional sugar-for-energy applications. Ultimately, we expect that our data will help promote the valorisation of unused biomass resources, create economic value, while contributing to the implementation of sustainable biorefining systems.

KW - Arundo donax

KW - biomass

KW - cell wall

KW - Cortaderia selloana

KW - marginal lands

KW - Miscanthus × giganteus

KW - Phragmites australis

KW - white-rot fungi

UR - http://www.scopus.com/inward/record.url?scp=85110512453&partnerID=8YFLogxK

U2 - 10.3389/fpls.2021.679966

DO - 10.3389/fpls.2021.679966

M3 - Article

C2 - 34276732

VL - 12

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

M1 - 679966

ER -

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