Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription

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Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription. / Sharma, Priyanka; Lioutas, Antonios; Fernandez-Fuentes, Narcis; Quilez, Javier; Carbonell-Caballero, José; Wright, Roni H. G.; Di Vona, Chiara; Le Dily, François; Schüller, Roland; Eick, Dirk; Oliva, Baldomero.

In: Molecular Cell, Vol. 73, No. 1, 03.01.2019, p. 84-96.

Research output: Contribution to journalArticle

Harvard

Sharma, P, Lioutas, A, Fernandez-Fuentes, N, Quilez, J, Carbonell-Caballero, J, Wright, RHG, Di Vona, C, Le Dily, F, Schüller, R, Eick, D & Oliva, B 2019, 'Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription' Molecular Cell, vol. 73, no. 1, pp. 84-96. https://doi.org/10.1016/j.molcel.2018.10.016

APA

Sharma, P., Lioutas, A., Fernandez-Fuentes, N., Quilez, J., Carbonell-Caballero, J., Wright, R. H. G., ... Oliva, B. (2019). Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription. Molecular Cell, 73(1), 84-96. https://doi.org/10.1016/j.molcel.2018.10.016

Vancouver

Sharma P, Lioutas A, Fernandez-Fuentes N, Quilez J, Carbonell-Caballero J, Wright RHG et al. Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription. Molecular Cell. 2019 Jan 3;73(1):84-96. https://doi.org/10.1016/j.molcel.2018.10.016

Author

Sharma, Priyanka ; Lioutas, Antonios ; Fernandez-Fuentes, Narcis ; Quilez, Javier ; Carbonell-Caballero, José ; Wright, Roni H. G. ; Di Vona, Chiara ; Le Dily, François ; Schüller, Roland ; Eick, Dirk ; Oliva, Baldomero. / Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription. In: Molecular Cell. 2019 ; Vol. 73, No. 1. pp. 84-96.

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@article{db9819891e5e47e191fba72dec8108ac,
title = "Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription",
abstract = "The post-translational modification of key residues at the C-terminal domain of RNA polymerase II (RNAP2-CTD) coordinates transcription, splicing, and RNA processing by modulating its capacity to act as a landing platform for a variety of protein complexes. Here, we identify a new modification at the CTD, the deimination of arginine and its conversion to citrulline by peptidyl arginine deiminase 2 (PADI2), an enzyme that has been associated with several diseases, including cancer. We show that, among PADI family members, only PADI2 citrullinates R1810 (Cit1810) at repeat 31 of the CTD. Depletion of PADI2 or loss of R1810 results in accumulation of RNAP2 at transcription start sites, reduced gene expression, and inhibition of cell proliferation. Cit1810 is needed for interaction with the P-TEFb (positive transcription elongation factor b) kinase complex and for its recruitment to chromatin. In this way, CTD-Cit1810 favors RNAP2 pause release and efficient transcription in breast cancer cells",
keywords = "RNA polymerase II CTD, citrullination, PADI2, arginine 1810, breast cancer cells, proximal promoter pausing, cell proliferation, P_TEFb complex",
author = "Priyanka Sharma and Antonios Lioutas and Narcis Fernandez-Fuentes and Javier Quilez and Jos{\'e} Carbonell-Caballero and Wright, {Roni H. G.} and {Di Vona}, Chiara and {Le Dily}, Fran{\cc}ois and Roland Sch{\"u}ller and Dirk Eick and Baldomero Oliva",
year = "2019",
month = "1",
day = "3",
doi = "10.1016/j.molcel.2018.10.016",
language = "English",
volume = "73",
pages = "84--96",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Elsevier",
number = "1",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription

AU - Sharma, Priyanka

AU - Lioutas, Antonios

AU - Fernandez-Fuentes, Narcis

AU - Quilez, Javier

AU - Carbonell-Caballero, José

AU - Wright, Roni H. G.

AU - Di Vona, Chiara

AU - Le Dily, François

AU - Schüller, Roland

AU - Eick, Dirk

AU - Oliva, Baldomero

PY - 2019/1/3

Y1 - 2019/1/3

N2 - The post-translational modification of key residues at the C-terminal domain of RNA polymerase II (RNAP2-CTD) coordinates transcription, splicing, and RNA processing by modulating its capacity to act as a landing platform for a variety of protein complexes. Here, we identify a new modification at the CTD, the deimination of arginine and its conversion to citrulline by peptidyl arginine deiminase 2 (PADI2), an enzyme that has been associated with several diseases, including cancer. We show that, among PADI family members, only PADI2 citrullinates R1810 (Cit1810) at repeat 31 of the CTD. Depletion of PADI2 or loss of R1810 results in accumulation of RNAP2 at transcription start sites, reduced gene expression, and inhibition of cell proliferation. Cit1810 is needed for interaction with the P-TEFb (positive transcription elongation factor b) kinase complex and for its recruitment to chromatin. In this way, CTD-Cit1810 favors RNAP2 pause release and efficient transcription in breast cancer cells

AB - The post-translational modification of key residues at the C-terminal domain of RNA polymerase II (RNAP2-CTD) coordinates transcription, splicing, and RNA processing by modulating its capacity to act as a landing platform for a variety of protein complexes. Here, we identify a new modification at the CTD, the deimination of arginine and its conversion to citrulline by peptidyl arginine deiminase 2 (PADI2), an enzyme that has been associated with several diseases, including cancer. We show that, among PADI family members, only PADI2 citrullinates R1810 (Cit1810) at repeat 31 of the CTD. Depletion of PADI2 or loss of R1810 results in accumulation of RNAP2 at transcription start sites, reduced gene expression, and inhibition of cell proliferation. Cit1810 is needed for interaction with the P-TEFb (positive transcription elongation factor b) kinase complex and for its recruitment to chromatin. In this way, CTD-Cit1810 favors RNAP2 pause release and efficient transcription in breast cancer cells

KW - RNA polymerase II CTD

KW - citrullination

KW - PADI2

KW - arginine 1810

KW - breast cancer cells

KW - proximal promoter pausing

KW - cell proliferation

KW - P_TEFb complex

U2 - 10.1016/j.molcel.2018.10.016

DO - 10.1016/j.molcel.2018.10.016

M3 - Article

VL - 73

SP - 84

EP - 96

JO - Molecular Cell

JF - Molecular Cell

SN - 1097-2765

IS - 1

ER -

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