Anti-inflammatory properties of an extract of M. ilicifolia in the human intestinal epithelial Caco-2 cell line

Ruth Wonfor; Manuela Natoli; Ifat Shah; Manfred Beckmann; Robert J. Nash; Deborah Nash

doi:10.1016/j.jep.2017.08.006

Anti-inflammatory properties of an extract of M. ilicifolia in the human intestinal epithelial Caco-2 cell line

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Anti-inflammatory properties of an extract of M. ilicifolia in the human intestinal epithelial Caco-2 cell line. / Wonfor, Ruth; Natoli, Manuela; Shah, Ifat et al.

In: Journal of Ethnopharmacology, Vol. 209, 14.09.2017, p. 283-287.

Research output: Contribution to journal › Article › peer-review

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@article{2f03b61268ba43b39f11ae04813fe892,

title = "Anti-inflammatory properties of an extract of M. ilicifolia in the human intestinal epithelial Caco-2 cell line",

abstract = "Ethnopharmacology relevanceMaytenus ilicifolia is a Celastracea plant used in traditional medicine to alleviate digestive tract inflammatory disorders.Aim of the studyWe investigated anti-inflammatory properties of M. ilicifolia crude extract towards Caco-2 cell line, as a model of Toll-like Receptor 2 (TLR-2) inflammatory pathway.Materials and MethodsToxicity was assessed following culture of Caco-2 with M. ilicifolia, using apparent cell permeability and trans-epithelial electric resistance. Anti-inflammatory properties of M. ilicifolia were assessed through IL-8 secretion and TLR-2 associated gene expression of Caco-2 cells with or without an LTA challenge.ResultsM. ilicifolia was not toxic to Caco-2 cells. M. ilicifolia down-regulated TLR2 expression with and without LTA challenge but had no effect on other genes. Following LTA challenge of Caco-2 cells, 100 and 200 µg/mL M. ilicifolia abrogated IL-8 secretion.ConclusionsWe provide preliminary data for some M. ilicifolia anti-inflammatory properties. Further research must establish the full extent and mode of action on particular inflammatory pathways",

keywords = "Anti-inflammatory, Caco-2 cells, Inflammation, M. ilicifolia, Toxicity",

author = "Ruth Wonfor and Manuela Natoli and Ifat Shah and Manfred Beckmann and Nash, {Robert J.} and Deborah Nash",

note = "Funding Information: We thank Dr Carol Michael for supplying freeze-dried M. ilicifolia for use in this study. This work was funded by a Technology Strategy Board Innovation Voucher. The authors gratefully acknowledge support from Biotechnology and Biological Sciences Research Council (BBSRC) Institute Strategic Programme Grant on Energy Grasses and Biorefining (BB/J0042/1) and BEACON Plus from the European Regional Development Fund (ERDF:80851) through the Welsh European Funding Office, part of the Welsh Assembly. Publisher Copyright: {\textcopyright} 2017 Elsevier Ireland Ltd",

year = "2017",

month = sep,

day = "14",

doi = "10.1016/j.jep.2017.08.006",

language = "English",

volume = "209",

pages = "283--287",

journal = "Journal of Ethnopharmacology",

issn = "0378-8741",

publisher = "Elsevier",

}

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TY - JOUR

T1 - Anti-inflammatory properties of an extract of M. ilicifolia in the human intestinal epithelial Caco-2 cell line

AU - Wonfor, Ruth

AU - Natoli, Manuela

AU - Shah, Ifat

AU - Beckmann, Manfred

AU - Nash, Robert J.

AU - Nash, Deborah

N1 - Funding Information: We thank Dr Carol Michael for supplying freeze-dried M. ilicifolia for use in this study. This work was funded by a Technology Strategy Board Innovation Voucher. The authors gratefully acknowledge support from Biotechnology and Biological Sciences Research Council (BBSRC) Institute Strategic Programme Grant on Energy Grasses and Biorefining (BB/J0042/1) and BEACON Plus from the European Regional Development Fund (ERDF:80851) through the Welsh European Funding Office, part of the Welsh Assembly. Publisher Copyright: © 2017 Elsevier Ireland Ltd

PY - 2017/9/14

Y1 - 2017/9/14

N2 - Ethnopharmacology relevanceMaytenus ilicifolia is a Celastracea plant used in traditional medicine to alleviate digestive tract inflammatory disorders.Aim of the studyWe investigated anti-inflammatory properties of M. ilicifolia crude extract towards Caco-2 cell line, as a model of Toll-like Receptor 2 (TLR-2) inflammatory pathway.Materials and MethodsToxicity was assessed following culture of Caco-2 with M. ilicifolia, using apparent cell permeability and trans-epithelial electric resistance. Anti-inflammatory properties of M. ilicifolia were assessed through IL-8 secretion and TLR-2 associated gene expression of Caco-2 cells with or without an LTA challenge.ResultsM. ilicifolia was not toxic to Caco-2 cells. M. ilicifolia down-regulated TLR2 expression with and without LTA challenge but had no effect on other genes. Following LTA challenge of Caco-2 cells, 100 and 200 µg/mL M. ilicifolia abrogated IL-8 secretion.ConclusionsWe provide preliminary data for some M. ilicifolia anti-inflammatory properties. Further research must establish the full extent and mode of action on particular inflammatory pathways

AB - Ethnopharmacology relevanceMaytenus ilicifolia is a Celastracea plant used in traditional medicine to alleviate digestive tract inflammatory disorders.Aim of the studyWe investigated anti-inflammatory properties of M. ilicifolia crude extract towards Caco-2 cell line, as a model of Toll-like Receptor 2 (TLR-2) inflammatory pathway.Materials and MethodsToxicity was assessed following culture of Caco-2 with M. ilicifolia, using apparent cell permeability and trans-epithelial electric resistance. Anti-inflammatory properties of M. ilicifolia were assessed through IL-8 secretion and TLR-2 associated gene expression of Caco-2 cells with or without an LTA challenge.ResultsM. ilicifolia was not toxic to Caco-2 cells. M. ilicifolia down-regulated TLR2 expression with and without LTA challenge but had no effect on other genes. Following LTA challenge of Caco-2 cells, 100 and 200 µg/mL M. ilicifolia abrogated IL-8 secretion.ConclusionsWe provide preliminary data for some M. ilicifolia anti-inflammatory properties. Further research must establish the full extent and mode of action on particular inflammatory pathways

KW - Anti-inflammatory

KW - Caco-2 cells

KW - Inflammation

KW - M. ilicifolia

KW - Toxicity

UR - http://www.scopus.com/inward/record.url?scp=85026920733&partnerID=8YFLogxK

U2 - 10.1016/j.jep.2017.08.006

DO - 10.1016/j.jep.2017.08.006

M3 - Article

C2 - 28789861

VL - 209

SP - 283