Anti-inflammatory properties of an extract of M. ilicifolia in the human intestinal epithelial Caco-2 cell line
Authors
Organisations
| Type | Article |
|---|
Permanent link
| Original language | English |
|---|---|
| Pages (from-to) | 283-287 |
| Number of pages | 5 |
| Journal | Journal of Ethnopharmacology |
| Volume | 209 |
| Early online date | 05 Aug 2017 |
| DOI | |
| Publication status | Published - 14 Sep 2017 |
Abstract
Ethnopharmacology relevance
Maytenus ilicifolia is a Celastracea plant used in traditional medicine to alleviate digestive tract inflammatory disorders.
Aim of the study
We investigated anti-inflammatory properties of M. ilicifolia crude extract towards Caco-2 cell line, as a model of Toll-like Receptor 2 (TLR-2) inflammatory pathway.
Materials and Methods
Toxicity was assessed following culture of Caco-2 with M. ilicifolia, using apparent cell permeability and trans-epithelial electric resistance. Anti-inflammatory properties of M. ilicifolia were assessed through IL-8 secretion and TLR-2 associated gene expression of Caco-2 cells with or without an LTA challenge.
Results
M. ilicifolia was not toxic to Caco-2 cells. M. ilicifolia down-regulated TLR2 expression with and without LTA challenge but had no effect on other genes. Following LTA challenge of Caco-2 cells, 100 and 200 µg/mL M. ilicifolia abrogated IL-8 secretion.
Conclusions
We provide preliminary data for some M. ilicifolia anti-inflammatory properties. Further research must establish the full extent and mode of action on particular inflammatory pathways
Maytenus ilicifolia is a Celastracea plant used in traditional medicine to alleviate digestive tract inflammatory disorders.
Aim of the study
We investigated anti-inflammatory properties of M. ilicifolia crude extract towards Caco-2 cell line, as a model of Toll-like Receptor 2 (TLR-2) inflammatory pathway.
Materials and Methods
Toxicity was assessed following culture of Caco-2 with M. ilicifolia, using apparent cell permeability and trans-epithelial electric resistance. Anti-inflammatory properties of M. ilicifolia were assessed through IL-8 secretion and TLR-2 associated gene expression of Caco-2 cells with or without an LTA challenge.
Results
M. ilicifolia was not toxic to Caco-2 cells. M. ilicifolia down-regulated TLR2 expression with and without LTA challenge but had no effect on other genes. Following LTA challenge of Caco-2 cells, 100 and 200 µg/mL M. ilicifolia abrogated IL-8 secretion.
Conclusions
We provide preliminary data for some M. ilicifolia anti-inflammatory properties. Further research must establish the full extent and mode of action on particular inflammatory pathways
Keywords
- inflammation, Caco-2 cells, M. ilicifolia, toxicity, anti-inflammatory
Documents
- Anti-inflammatory properties of an extract of M. ilicifolia in the human intestianl epthelial Caco-2 cell line
Accepted author manuscript, 770 KB, PDF