An Analytical Pipeline for Quantitative Characterization of Dietary IntakeApplication To Assess Grape Intake

Authors Organisations
  • Isabel Garcia-Perez(Author)
    Imperial College London
  • Joram M Posma(Author)
    Imperial College London
  • Edward S. Chambers(Author)
    Imperial College London
  • Jeremy K. Nicholson(Author)
    Imperial College London
  • John C. Mathers(Author)
    Newcastle University
  • Manfred Beckmann(Author)
  • John Draper(Author)
  • Elaine Holmes(Author)
    Imperial College London
  • Gary Frost(Author)
    Imperial College London
Type Article
Original languageEnglish
Pages (from-to)2423-2431
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Issue number11
Early online date24 Feb 2016
Publication statusPublished - 23 Mar 2016
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Lack of accurate dietary assessment in free-living populations requires discovery of new biomarkers reflecting food intake qualitatively and quantitatively to objectively evaluate effects of diet on health. We provide a proof-of-principle for an analytical pipeline to identify quantitative dietary biomarkers. Tartaric acid was identified by nuclear magnetic resonance spectroscopy as a dose-responsive urinary biomarker of grape intake and subsequently quantified in volunteers following a series of 4-day dietary interventions incorporating 0 g/day, 50 g/day, 100 g/day, and 150 g/day of grapes in standardized diets from a randomized controlled clinical trial. Most accurate quantitative predictions of grape intake were obtained in 24 h urine samples which have the strongest linear relationship between grape intake and tartaric acid excretion (r(2) = 0.90). This new methodological pipeline for estimating nutritional intake based on coupling dietary intake information and quantified nutritional biomarkers was developed and validated in a controlled dietary intervention study, showing that this approach can improve the accuracy of estimating nutritional intakes.


  • accurate dietary assessment, metabolic profiling, nutritional intake, quantified dietary biomarkers, tartaric acid