A Standardized Strategy for Simultaneous Quantification of Urine Metabolites to Validate Development of a Biomarker Panel Allowing Comprehensive Assessment of Dietary Exposure

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A Standardized Strategy for Simultaneous Quantification of Urine Metabolites to Validate Development of a Biomarker Panel Allowing Comprehensive Assessment of Dietary Exposure. / Beckmann, Manfred; Wilson, Thomas; Zubair, Hassan; Lloyd, Amanda J.; Lyons, Laura; Phillips, Helen; Tailliart, Kathleen; Gregory, Nicholas; Thatcher, Rhys; Garcia-Perez, Isabel; Frost, Gary; Mathers, John M.; Draper, John.

In: Molecular Nutrition and Food Research, 28.09.2020.

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@article{c9309c6e51e2483e8e4174548d2c43c5,
title = "A Standardized Strategy for Simultaneous Quantification of Urine Metabolites to Validate Development of a Biomarker Panel Allowing Comprehensive Assessment of Dietary Exposure",
abstract = "ScopeMetabolites derived from individual foods found in human biofluids after consumption could provide objective measures of dietary intake. For comprehensive dietary assessment, quantification methods would need to manage the structurally diverse mixture of target metabolites present at wide concentration ranges.Methods and resultsA strategy for selection of candidate dietary exposure biomarkers is developed. An analytical method for 62 food biomarkers is validated by extensive analysis of chromatographic and ionization behavior characteristics using triple quadrupole mass spectrometry. Urine samples from two food intervention studies are used: a controlled, inpatient study (n = 19) and a free‐living study where individuals (n = 15) are provided with food as a series of menu plans. As proof‐of‐principle, it is demonstrated that the biomarker panel could discriminate between menu plans by detecting distinctive changes in the concentration in urine of targeted metabolites. Quantitative relationships between four biomarker concentrations in urine and dietary intake are shown.ConclusionDesign concepts for an analytical strategy are demonstrated, allowing simultaneous quantification of a comprehensive panel of chemically‐diverse biomarkers of a wide range of commonly‐consumed foods. It is proposed that integration of self‐reported dietary recording tools with biomarker approaches will provide more robust assessment of dietary exposure.",
keywords = "dietary biomarkers, liquid chromatography mass spectrometry, metabolomics, targeted quantification, urine",
author = "Manfred Beckmann and Thomas Wilson and Hassan Zubair and Lloyd, {Amanda J.} and Laura Lyons and Helen Phillips and Kathleen Tailliart and Nicholas Gregory and Rhys Thatcher and Isabel Garcia-Perez and Gary Frost and Mathers, {John M.} and John Draper",
note = "Funding: MR/ J010308/1",
year = "2020",
month = sep,
day = "28",
doi = "10.1002/mnfr.202000517",
language = "English",
journal = "Molecular Nutrition and Food Research",
issn = "1613-4125",
publisher = "Wiley",

}

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TY - JOUR

T1 - A Standardized Strategy for Simultaneous Quantification of Urine Metabolites to Validate Development of a Biomarker Panel Allowing Comprehensive Assessment of Dietary Exposure

AU - Beckmann, Manfred

AU - Wilson, Thomas

AU - Zubair, Hassan

AU - Lloyd, Amanda J.

AU - Lyons, Laura

AU - Phillips, Helen

AU - Tailliart, Kathleen

AU - Gregory, Nicholas

AU - Thatcher, Rhys

AU - Garcia-Perez, Isabel

AU - Frost, Gary

AU - Mathers, John M.

AU - Draper, John

N1 - Funding: MR/ J010308/1

PY - 2020/9/28

Y1 - 2020/9/28

N2 - ScopeMetabolites derived from individual foods found in human biofluids after consumption could provide objective measures of dietary intake. For comprehensive dietary assessment, quantification methods would need to manage the structurally diverse mixture of target metabolites present at wide concentration ranges.Methods and resultsA strategy for selection of candidate dietary exposure biomarkers is developed. An analytical method for 62 food biomarkers is validated by extensive analysis of chromatographic and ionization behavior characteristics using triple quadrupole mass spectrometry. Urine samples from two food intervention studies are used: a controlled, inpatient study (n = 19) and a free‐living study where individuals (n = 15) are provided with food as a series of menu plans. As proof‐of‐principle, it is demonstrated that the biomarker panel could discriminate between menu plans by detecting distinctive changes in the concentration in urine of targeted metabolites. Quantitative relationships between four biomarker concentrations in urine and dietary intake are shown.ConclusionDesign concepts for an analytical strategy are demonstrated, allowing simultaneous quantification of a comprehensive panel of chemically‐diverse biomarkers of a wide range of commonly‐consumed foods. It is proposed that integration of self‐reported dietary recording tools with biomarker approaches will provide more robust assessment of dietary exposure.

AB - ScopeMetabolites derived from individual foods found in human biofluids after consumption could provide objective measures of dietary intake. For comprehensive dietary assessment, quantification methods would need to manage the structurally diverse mixture of target metabolites present at wide concentration ranges.Methods and resultsA strategy for selection of candidate dietary exposure biomarkers is developed. An analytical method for 62 food biomarkers is validated by extensive analysis of chromatographic and ionization behavior characteristics using triple quadrupole mass spectrometry. Urine samples from two food intervention studies are used: a controlled, inpatient study (n = 19) and a free‐living study where individuals (n = 15) are provided with food as a series of menu plans. As proof‐of‐principle, it is demonstrated that the biomarker panel could discriminate between menu plans by detecting distinctive changes in the concentration in urine of targeted metabolites. Quantitative relationships between four biomarker concentrations in urine and dietary intake are shown.ConclusionDesign concepts for an analytical strategy are demonstrated, allowing simultaneous quantification of a comprehensive panel of chemically‐diverse biomarkers of a wide range of commonly‐consumed foods. It is proposed that integration of self‐reported dietary recording tools with biomarker approaches will provide more robust assessment of dietary exposure.

KW - dietary biomarkers

KW - liquid chromatography mass spectrometry

KW - metabolomics

KW - targeted quantification

KW - urine

UR - http://www.scopus.com/inward/record.url?scp=85091476733&partnerID=8YFLogxK

U2 - 10.1002/mnfr.202000517

DO - 10.1002/mnfr.202000517

M3 - Article

C2 - 32926540

AN - SCOPUS:85091476733

JO - Molecular Nutrition and Food Research

JF - Molecular Nutrition and Food Research

SN - 1613-4125

M1 - 2000517

ER -

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