A Standardized Strategy for Simultaneous Quantification of Urine Metabolites to Validate Development of a Biomarker Panel Allowing Comprehensive Assessment of Dietary Exposure
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A Standardized Strategy for Simultaneous Quantification of Urine Metabolites to Validate Development of a Biomarker Panel Allowing Comprehensive Assessment of Dietary Exposure. / Beckmann, Manfred; Wilson, Thomas; Zubair, Hassan et al.
In: Molecular Nutrition and Food Research, 28.09.2020.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - A Standardized Strategy for Simultaneous Quantification of Urine Metabolites to Validate Development of a Biomarker Panel Allowing Comprehensive Assessment of Dietary Exposure
AU - Beckmann, Manfred
AU - Wilson, Thomas
AU - Zubair, Hassan
AU - Lloyd, Amanda J.
AU - Lyons, Laura
AU - Phillips, Helen
AU - Tailliart, Kathleen
AU - Gregory, Nicholas
AU - Thatcher, Rhys
AU - Garcia-Perez, Isabel
AU - Frost, Gary
AU - Mathers, John M.
AU - Draper, John
N1 - Funding: MR/ J010308/1
PY - 2020/9/28
Y1 - 2020/9/28
N2 - ScopeMetabolites derived from individual foods found in human biofluids after consumption could provide objective measures of dietary intake. For comprehensive dietary assessment, quantification methods would need to manage the structurally diverse mixture of target metabolites present at wide concentration ranges.Methods and resultsA strategy for selection of candidate dietary exposure biomarkers is developed. An analytical method for 62 food biomarkers is validated by extensive analysis of chromatographic and ionization behavior characteristics using triple quadrupole mass spectrometry. Urine samples from two food intervention studies are used: a controlled, inpatient study (n = 19) and a free‐living study where individuals (n = 15) are provided with food as a series of menu plans. As proof‐of‐principle, it is demonstrated that the biomarker panel could discriminate between menu plans by detecting distinctive changes in the concentration in urine of targeted metabolites. Quantitative relationships between four biomarker concentrations in urine and dietary intake are shown.ConclusionDesign concepts for an analytical strategy are demonstrated, allowing simultaneous quantification of a comprehensive panel of chemically‐diverse biomarkers of a wide range of commonly‐consumed foods. It is proposed that integration of self‐reported dietary recording tools with biomarker approaches will provide more robust assessment of dietary exposure.
AB - ScopeMetabolites derived from individual foods found in human biofluids after consumption could provide objective measures of dietary intake. For comprehensive dietary assessment, quantification methods would need to manage the structurally diverse mixture of target metabolites present at wide concentration ranges.Methods and resultsA strategy for selection of candidate dietary exposure biomarkers is developed. An analytical method for 62 food biomarkers is validated by extensive analysis of chromatographic and ionization behavior characteristics using triple quadrupole mass spectrometry. Urine samples from two food intervention studies are used: a controlled, inpatient study (n = 19) and a free‐living study where individuals (n = 15) are provided with food as a series of menu plans. As proof‐of‐principle, it is demonstrated that the biomarker panel could discriminate between menu plans by detecting distinctive changes in the concentration in urine of targeted metabolites. Quantitative relationships between four biomarker concentrations in urine and dietary intake are shown.ConclusionDesign concepts for an analytical strategy are demonstrated, allowing simultaneous quantification of a comprehensive panel of chemically‐diverse biomarkers of a wide range of commonly‐consumed foods. It is proposed that integration of self‐reported dietary recording tools with biomarker approaches will provide more robust assessment of dietary exposure.
KW - dietary biomarkers
KW - liquid chromatography mass spectrometry
KW - metabolomics
KW - targeted quantification
KW - urine
UR - http://www.scopus.com/inward/record.url?scp=85091476733&partnerID=8YFLogxK
U2 - 10.1002/mnfr.202000517
DO - 10.1002/mnfr.202000517
M3 - Article
C2 - 32926540
AN - SCOPUS:85091476733
JO - Molecular Nutrition and Food Research
JF - Molecular Nutrition and Food Research
SN - 1613-4125
M1 - 2000517
ER -