A route to decreasing N pollution from livestockUse of Festulolium hybrids improves efficiency of N flows in rumen simulation fermenters

Standard

A route to decreasing N pollution from livestock : Use of Festulolium hybrids improves efficiency of N flows in rumen simulation fermenters. / Wanjiru, Stephen Kamau; Belanche Gracia, Alejandro; Davies, Terri; Rees Stevens, Pauline; Humphreys, Mike; Kingston-Smith, Alison.

In: Food and Energy Security, 22.05.2020.

Research output: Contribution to journalArticle

Bibtex - Download

@article{dcc64466f36e4022bf1acd1f158ac4f9,
title = "A route to decreasing N pollution from livestock: Use of Festulolium hybrids improves efficiency of N flows in rumen simulation fermenters",
abstract = "Ruminant agriculture suffers from inefficient capture of forage protein and consequential release of N pollutants to land. This is due to proteolysis in the rumen catalyzed by both microbial but initially endogenous plant proteases. Plant breeding‐based solutions are sought to minimize these negative environmental impacts. The aim of this study was to perform an integrated study of rumen N metabolism using semi‐continuous rumen simulation fermenters (Rusitec) to explore the extent to which swards containing Festulolium populations (interspecific hybrids between Lolium and Festuca grass species) with decreased rates of endogenous protein degradation conferred advantageous protein utilization in comparison with a National Listed perennial ryegrass. An in vitro experiment was conducted using three Festulolium hybrids (Lolium perenne × Festuca arundinacea var. glaucescens , LpFg; Lolium perenne × Festuca mairei , LpFm; and Lolium multiflorum × Festuca arundinacea var. glaucescens , LmFg) and a Lolium perenne , Lp control. LpFm and LmFg demonstrated significantly lower plant‐mediated proteolysis than the control. Fresh forage was incubated in Rusitec with rumen fluid from four donor cows. Feed disappearance and production of gas, methane, and volatile fatty acids were similar across cultivars. Whereas no differences in microbial protein synthesis were noted across treatments during early fermentation (0–6 hr after feeding), an increased microbial N flow in LpFm (+30%) and LmFg hybrids (+41%) was observed during late fermentation (6–24 hr after feeding), with higher overall microbial N flows (+13.5% and + 20.2%, respectively) compared with the control (Lp). We propose an underpinning mechanism involving the partitioning of amino acid catabolism toward branched‐chain amino acids and microbial protein synthesis in grasses with slow plant‐mediated proteolysis instead of accumulation of rumen ammonia in grasses with fast plant‐mediated proteolysis. These observations indicate the potential of Festulolium hybrids with a slow plant‐mediated proteolysis trait to improve the efficiency of capture of forage protein and decrease the release of N pollutants onto the land",
keywords = "festuca, festulolium, lolium, nitrogen use efficiency, protein, proteolysis, rumen",
author = "Wanjiru, {Stephen Kamau} and {Belanche Gracia}, Alejandro and Terri Davies and {Rees Stevens}, Pauline and Mike Humphreys and Alison Kingston-Smith",
year = "2020",
month = may,
day = "22",
doi = "10.1002/fes3.209",
language = "English",
journal = "Food and Energy Security",
issn = "2048-3694",
publisher = "Wiley",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - A route to decreasing N pollution from livestock

T2 - Use of Festulolium hybrids improves efficiency of N flows in rumen simulation fermenters

AU - Wanjiru, Stephen Kamau

AU - Belanche Gracia, Alejandro

AU - Davies, Terri

AU - Rees Stevens, Pauline

AU - Humphreys, Mike

AU - Kingston-Smith, Alison

PY - 2020/5/22

Y1 - 2020/5/22

N2 - Ruminant agriculture suffers from inefficient capture of forage protein and consequential release of N pollutants to land. This is due to proteolysis in the rumen catalyzed by both microbial but initially endogenous plant proteases. Plant breeding‐based solutions are sought to minimize these negative environmental impacts. The aim of this study was to perform an integrated study of rumen N metabolism using semi‐continuous rumen simulation fermenters (Rusitec) to explore the extent to which swards containing Festulolium populations (interspecific hybrids between Lolium and Festuca grass species) with decreased rates of endogenous protein degradation conferred advantageous protein utilization in comparison with a National Listed perennial ryegrass. An in vitro experiment was conducted using three Festulolium hybrids (Lolium perenne × Festuca arundinacea var. glaucescens , LpFg; Lolium perenne × Festuca mairei , LpFm; and Lolium multiflorum × Festuca arundinacea var. glaucescens , LmFg) and a Lolium perenne , Lp control. LpFm and LmFg demonstrated significantly lower plant‐mediated proteolysis than the control. Fresh forage was incubated in Rusitec with rumen fluid from four donor cows. Feed disappearance and production of gas, methane, and volatile fatty acids were similar across cultivars. Whereas no differences in microbial protein synthesis were noted across treatments during early fermentation (0–6 hr after feeding), an increased microbial N flow in LpFm (+30%) and LmFg hybrids (+41%) was observed during late fermentation (6–24 hr after feeding), with higher overall microbial N flows (+13.5% and + 20.2%, respectively) compared with the control (Lp). We propose an underpinning mechanism involving the partitioning of amino acid catabolism toward branched‐chain amino acids and microbial protein synthesis in grasses with slow plant‐mediated proteolysis instead of accumulation of rumen ammonia in grasses with fast plant‐mediated proteolysis. These observations indicate the potential of Festulolium hybrids with a slow plant‐mediated proteolysis trait to improve the efficiency of capture of forage protein and decrease the release of N pollutants onto the land

AB - Ruminant agriculture suffers from inefficient capture of forage protein and consequential release of N pollutants to land. This is due to proteolysis in the rumen catalyzed by both microbial but initially endogenous plant proteases. Plant breeding‐based solutions are sought to minimize these negative environmental impacts. The aim of this study was to perform an integrated study of rumen N metabolism using semi‐continuous rumen simulation fermenters (Rusitec) to explore the extent to which swards containing Festulolium populations (interspecific hybrids between Lolium and Festuca grass species) with decreased rates of endogenous protein degradation conferred advantageous protein utilization in comparison with a National Listed perennial ryegrass. An in vitro experiment was conducted using three Festulolium hybrids (Lolium perenne × Festuca arundinacea var. glaucescens , LpFg; Lolium perenne × Festuca mairei , LpFm; and Lolium multiflorum × Festuca arundinacea var. glaucescens , LmFg) and a Lolium perenne , Lp control. LpFm and LmFg demonstrated significantly lower plant‐mediated proteolysis than the control. Fresh forage was incubated in Rusitec with rumen fluid from four donor cows. Feed disappearance and production of gas, methane, and volatile fatty acids were similar across cultivars. Whereas no differences in microbial protein synthesis were noted across treatments during early fermentation (0–6 hr after feeding), an increased microbial N flow in LpFm (+30%) and LmFg hybrids (+41%) was observed during late fermentation (6–24 hr after feeding), with higher overall microbial N flows (+13.5% and + 20.2%, respectively) compared with the control (Lp). We propose an underpinning mechanism involving the partitioning of amino acid catabolism toward branched‐chain amino acids and microbial protein synthesis in grasses with slow plant‐mediated proteolysis instead of accumulation of rumen ammonia in grasses with fast plant‐mediated proteolysis. These observations indicate the potential of Festulolium hybrids with a slow plant‐mediated proteolysis trait to improve the efficiency of capture of forage protein and decrease the release of N pollutants onto the land

KW - festuca

KW - festulolium

KW - lolium

KW - nitrogen use efficiency

KW - protein

KW - proteolysis

KW - rumen

U2 - 10.1002/fes3.209

DO - 10.1002/fes3.209

M3 - Article

C2 - 32999716

JO - Food and Energy Security

JF - Food and Energy Security

SN - 2048-3694

M1 - e209

ER -

Show download statistics
View graph of relations
Citation formats