A Proteomics Approach To Quantify Protein Levels Following RNA InterferenceCase Study with Glutathione Transferase Superfamily from the Model Metazoan Caenorhabditis elegans

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Type Article
Original languageEnglish
Pages (from-to)3314-3318
Number of pages5
JournalJournal of Proteome Research
Volume7
Issue number8
DOI
Publication statusPublished - 27 Jun 2008
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Abstract

Loss-of-function phenotypic analysis via interference RNA (RNAi) technology is a revolutionary approach to assigning gene function. While transcript-based methodologies commonly validate RNAi gene suppression investigations; protein-based validation is less developed. This report illustrates the potential for two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-DE) and gel analysis to quantify protein levels following RNAi. This case study involves three glutathione transferase (GST) genes targeted by RNAi from the model organism Caenorhabditis elegans.

Keywords

  • RNAi, Glutathione transferase, Caenorhabditis elegans