A metagenomic snapshot of taxonomic and functional diversity in an Alpine glacier cryoconite ecosystemAlpine cryoconite metagenome

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@article{f9953fc5d58c42838b1ec7dbea455341,
title = "A metagenomic snapshot of taxonomic and functional diversity in an Alpine glacier cryoconite ecosystem: Alpine cryoconite metagenome",
abstract = "Cryoconite is a microbe-mineral aggregate which darkens the ice surface of glaciers. Microbial process and marker gene PCR-dependent measurements reveal active and diverse cryoconite microbial communities on Polar glaciers. Here we provide the first report of a cryoconite metagenome and culture-independent study of Alpine cryoconite microbial diversity. We assembled 1.2 Gbp of metagenomic DNA sequenced using a Illumina HiScanSQ from cryoconite holes across the ablation zone of Rotmoosferner in the Austrian Alps. The metagenome revealed a bacterially-dominated community, with Proteobacteria (62% of bacterial-assigned contigs) and Bacteroidetes (14%) considerably more abundant than Cyanobacteria (2.5%). Streptophyte DNA dominated the eukaryotic metagenome. Functional genes linked to N, Fe, S and P cycling illustrated an acquisitive trend and a nitrogen cycle based upon efficient ammonia recycling. A comparison of 32 metagenome datasets revealed a similarity in functional profiles between the cryoconite and metagenones characterized from other cold microbe-mineral aggregates. Overall, the metagenomic snapshot reveals the cryoconite ecosystem of this Alpine glacier as dependent on scavenging carbon and nutrients from allochthonous sources, in particular mosses transported by wind from ice-marginal habitats, consistent with net heterotrophy indicated by productivity measurements. A transition from singular snapshots of cryoconite metagenomes to comparative analyses is advocated.",
author = "Arwyn Edwards and Pachebat, {Justin Alexander} and Swain, {Martin Thomas} and Hegarty, {Matthew John} and Hodson, {Andrew J.} and Irvine-Fynn, {Tristram David} and {Edwards Rassner}, {Sara Maria} and Birgit Sattler",
note = "Edwards, A., Pachebat, J. A., Swain, M. T., Hegarty, M. J., Hodson, A. J., Irvine-Fynn, T. D., Edwards Rassner, S. M., Sattler, B. (2013). A metagenomic snapshot of taxonomic and functional diversity in an Alpine glacier cryoconite ecosystem: Alpine cryoconite metagenome. Environmental Research Letters, 8 (3).",
year = "2013",
doi = "10.1088/1748-9326/8/3/035003",
language = "English",
volume = "8",
journal = "Environmental Research Letters",
issn = "1748-9326",
publisher = "IOP Publishing",
number = "3",

}

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TY - JOUR

T1 - A metagenomic snapshot of taxonomic and functional diversity in an Alpine glacier cryoconite ecosystem

T2 - Alpine cryoconite metagenome

AU - Edwards, Arwyn

AU - Pachebat, Justin Alexander

AU - Swain, Martin Thomas

AU - Hegarty, Matthew John

AU - Hodson, Andrew J.

AU - Irvine-Fynn, Tristram David

AU - Edwards Rassner, Sara Maria

AU - Sattler, Birgit

N1 - Edwards, A., Pachebat, J. A., Swain, M. T., Hegarty, M. J., Hodson, A. J., Irvine-Fynn, T. D., Edwards Rassner, S. M., Sattler, B. (2013). A metagenomic snapshot of taxonomic and functional diversity in an Alpine glacier cryoconite ecosystem: Alpine cryoconite metagenome. Environmental Research Letters, 8 (3).

PY - 2013

Y1 - 2013

N2 - Cryoconite is a microbe-mineral aggregate which darkens the ice surface of glaciers. Microbial process and marker gene PCR-dependent measurements reveal active and diverse cryoconite microbial communities on Polar glaciers. Here we provide the first report of a cryoconite metagenome and culture-independent study of Alpine cryoconite microbial diversity. We assembled 1.2 Gbp of metagenomic DNA sequenced using a Illumina HiScanSQ from cryoconite holes across the ablation zone of Rotmoosferner in the Austrian Alps. The metagenome revealed a bacterially-dominated community, with Proteobacteria (62% of bacterial-assigned contigs) and Bacteroidetes (14%) considerably more abundant than Cyanobacteria (2.5%). Streptophyte DNA dominated the eukaryotic metagenome. Functional genes linked to N, Fe, S and P cycling illustrated an acquisitive trend and a nitrogen cycle based upon efficient ammonia recycling. A comparison of 32 metagenome datasets revealed a similarity in functional profiles between the cryoconite and metagenones characterized from other cold microbe-mineral aggregates. Overall, the metagenomic snapshot reveals the cryoconite ecosystem of this Alpine glacier as dependent on scavenging carbon and nutrients from allochthonous sources, in particular mosses transported by wind from ice-marginal habitats, consistent with net heterotrophy indicated by productivity measurements. A transition from singular snapshots of cryoconite metagenomes to comparative analyses is advocated.

AB - Cryoconite is a microbe-mineral aggregate which darkens the ice surface of glaciers. Microbial process and marker gene PCR-dependent measurements reveal active and diverse cryoconite microbial communities on Polar glaciers. Here we provide the first report of a cryoconite metagenome and culture-independent study of Alpine cryoconite microbial diversity. We assembled 1.2 Gbp of metagenomic DNA sequenced using a Illumina HiScanSQ from cryoconite holes across the ablation zone of Rotmoosferner in the Austrian Alps. The metagenome revealed a bacterially-dominated community, with Proteobacteria (62% of bacterial-assigned contigs) and Bacteroidetes (14%) considerably more abundant than Cyanobacteria (2.5%). Streptophyte DNA dominated the eukaryotic metagenome. Functional genes linked to N, Fe, S and P cycling illustrated an acquisitive trend and a nitrogen cycle based upon efficient ammonia recycling. A comparison of 32 metagenome datasets revealed a similarity in functional profiles between the cryoconite and metagenones characterized from other cold microbe-mineral aggregates. Overall, the metagenomic snapshot reveals the cryoconite ecosystem of this Alpine glacier as dependent on scavenging carbon and nutrients from allochthonous sources, in particular mosses transported by wind from ice-marginal habitats, consistent with net heterotrophy indicated by productivity measurements. A transition from singular snapshots of cryoconite metagenomes to comparative analyses is advocated.

UR - http://hdl.handle.net/2160/10776

U2 - 10.1088/1748-9326/8/3/035003

DO - 10.1088/1748-9326/8/3/035003

M3 - Article

VL - 8

JO - Environmental Research Letters

JF - Environmental Research Letters

SN - 1748-9326

IS - 3

M1 - 035003

ER -

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