A defined antigen skin test for the diagnosis of bovine tuberculosis

Sreenidhi Srinivasan; Gareth Jones; Maroudam Veerasami; Sabine Steinbach; Thomas Holder; Aboma Zewude; Abebe Fromsa; Gobena Ameni; Laurel Easterling; Douwe Bakker; Nicholas Jeuleff; Glen Gifford; Glyn Hewinson; Hans-Martin Vordermeier; Vivek Kapur

doi:10.1126/sciadv.aax4899

A defined antigen skin test for the diagnosis of bovine tuberculosis

Standard

A defined antigen skin test for the diagnosis of bovine tuberculosis. / Srinivasan, Sreenidhi; Jones, Gareth; Veerasami, Maroudam et al.

In: Science Advances, Vol. 5, No. 7, eaax4899, 17.07.2019.

Research output: Contribution to journal › Article › peer-review

Harvard

Srinivasan, S, Jones, G, Veerasami, M, Steinbach, S, Holder, T, Zewude, A, Fromsa, A, Ameni, G, Easterling, L, Bakker, D, Jeuleff, N, Gifford, G, Hewinson, G, Vordermeier, H-M & Kapur, V 2019, 'A defined antigen skin test for the diagnosis of bovine tuberculosis', Science Advances, vol. 5, no. 7, eaax4899. https://doi.org/10.1126/sciadv.aax4899

APA

Srinivasan, S., Jones, G., Veerasami, M., Steinbach, S., Holder, T., Zewude, A., Fromsa, A., Ameni, G., Easterling, L., Bakker, D., Jeuleff, N., Gifford, G., Hewinson, G., Vordermeier, H-M., & Kapur, V. (2019). A defined antigen skin test for the diagnosis of bovine tuberculosis. Science Advances, 5(7), [eaax4899]. https://doi.org/10.1126/sciadv.aax4899

Vancouver

Srinivasan S, Jones G, Veerasami M, Steinbach S, Holder T, Zewude A et al. A defined antigen skin test for the diagnosis of bovine tuberculosis. Science Advances. 2019 Jul 17;5(7):eaax4899. doi: 10.1126/sciadv.aax4899

Author

Srinivasan, Sreenidhi ; Jones, Gareth ; Veerasami, Maroudam et al. / A defined antigen skin test for the diagnosis of bovine tuberculosis. In: Science Advances. 2019 ; Vol. 5, No. 7.

Bibtex - Download

@article{6ac7318949904171a6a4b734ca615f60,

title = "A defined antigen skin test for the diagnosis of bovine tuberculosis",

abstract = "Bovine tuberculosis (bTB) is a major zoonotic disease of cattle that is endemic in much of the world, limiting livestock productivity and representing a global public health threat. Because the standard tuberculin skin test precludes implementation of Bacille Calmette-Gu{\'e}rin (BCG) vaccine–based control programs, we here developed and evaluated a novel peptide-based defined antigen skin test (DST) to diagnose bTB and to differentiate infected from vaccinated animals (DIVA). The results, in laboratory assays and in experimentally or naturally infected animals, demonstrate that the peptide-based DST provides DIVA capability and equal or superior performance over the extant standard tuberculin surveillance test. Together with the ease of chemical synthesis, quality control, and lower burden for regulatory approval compared with recombinant antigens, the results of our studies show that the DST considerably improves a century-old standard and enables the development and implementation of critically needed surveillance and vaccination programs to accelerate bTB control.",

author = "Sreenidhi Srinivasan and Gareth Jones and Maroudam Veerasami and Sabine Steinbach and Thomas Holder and Aboma Zewude and Abebe Fromsa and Gobena Ameni and Laurel Easterling and Douwe Bakker and Nicholas Jeuleff and Glen Gifford and Glyn Hewinson and Hans-Martin Vordermeier and Vivek Kapur",

year = "2019",

month = jul,

day = "17",

doi = "10.1126/sciadv.aax4899",

language = "English",

volume = "5",

journal = "Science Advances",

issn = "2375-2548",

publisher = "American Association for the Advancement of Science",

number = "7",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - A defined antigen skin test for the diagnosis of bovine tuberculosis

AU - Srinivasan, Sreenidhi

AU - Jones, Gareth

AU - Veerasami, Maroudam

AU - Steinbach, Sabine

AU - Holder, Thomas

AU - Zewude, Aboma

AU - Fromsa, Abebe

AU - Ameni, Gobena

AU - Easterling, Laurel

AU - Bakker, Douwe

AU - Jeuleff, Nicholas

AU - Gifford, Glen

AU - Hewinson, Glyn

AU - Vordermeier, Hans-Martin

AU - Kapur, Vivek

PY - 2019/7/17

Y1 - 2019/7/17

N2 - Bovine tuberculosis (bTB) is a major zoonotic disease of cattle that is endemic in much of the world, limiting livestock productivity and representing a global public health threat. Because the standard tuberculin skin test precludes implementation of Bacille Calmette-Guérin (BCG) vaccine–based control programs, we here developed and evaluated a novel peptide-based defined antigen skin test (DST) to diagnose bTB and to differentiate infected from vaccinated animals (DIVA). The results, in laboratory assays and in experimentally or naturally infected animals, demonstrate that the peptide-based DST provides DIVA capability and equal or superior performance over the extant standard tuberculin surveillance test. Together with the ease of chemical synthesis, quality control, and lower burden for regulatory approval compared with recombinant antigens, the results of our studies show that the DST considerably improves a century-old standard and enables the development and implementation of critically needed surveillance and vaccination programs to accelerate bTB control.

AB - Bovine tuberculosis (bTB) is a major zoonotic disease of cattle that is endemic in much of the world, limiting livestock productivity and representing a global public health threat. Because the standard tuberculin skin test precludes implementation of Bacille Calmette-Guérin (BCG) vaccine–based control programs, we here developed and evaluated a novel peptide-based defined antigen skin test (DST) to diagnose bTB and to differentiate infected from vaccinated animals (DIVA). The results, in laboratory assays and in experimentally or naturally infected animals, demonstrate that the peptide-based DST provides DIVA capability and equal or superior performance over the extant standard tuberculin surveillance test. Together with the ease of chemical synthesis, quality control, and lower burden for regulatory approval compared with recombinant antigens, the results of our studies show that the DST considerably improves a century-old standard and enables the development and implementation of critically needed surveillance and vaccination programs to accelerate bTB control.

UR - https://advances.sciencemag.org/content/suppl/2019/07/15/5.7.eaax4899.DC1

U2 - 10.1126/sciadv.aax4899

DO - 10.1126/sciadv.aax4899

M3 - Article

C2 - 31328169

VL - 5

JO - Science Advances

JF - Science Advances

SN - 2375-2548

IS - 7

M1 - eaax4899

ER -