Visualisation of plastid degradation in sperm cells of wheat pollen

Awduron Sefydliadau
  • Lucia F. Primavesi(Awdur)
    Rothamsted Research
  • Huixia Wu(Awdur)
    Rothamsted Research
    Dow AgroSciences
  • Elisabeth A Mudd(Awdur)
    The University of Manchester
  • Anil Day(Awdur)
    The University of Manchester
  • Huw Jones(Awdur)
Math Erthygl
Iaith wreiddiolSaesneg
Tudalennau (o-i)229-237
CyfnodolynProtoplasma
Cyfrol254
Rhif y cyfnodolyn1
Dyddiad ar-lein cynnar21 Ion 2016
Dangosyddion eitem ddigidol (DOIs)
StatwsCyhoeddwyd - 01 Ion 2017
Cysylltiadau
Cysylltiad parhaol
Arddangos ystadegau lawrlwytho
Gweld graff cysylltiadau
Fformatau enwi

Crynodeb

Like most angiosperms, wheat (Triticum aestivum) shows maternal inheritance of plastids. It is thought that this takes place by cytoplasmic stripping at fertilisation rather than the absence of plastids in sperm cells. To determine the fate of plastids during sperm cell development, plastid-targeted green fluorescent protein was used to visualise these organelles in nuclear transgenic wheat lines. Fewer than thirty small 1-2-μm plastids were visible in early uninucleate pollen cells. These dramatically increased to several hundred larger (4 μm) plastids during pollen maturation and went through distinct morphological changes. Only small plastids were visible in generative cells (n = 25) and young sperm cells (n = 9). In mature sperm cells, these green fluorescent protein (GFP)-tagged plastids were absent. This is consistent with maternal inheritance of plastids resulting from their degradation in mature sperm cells in wheat.

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