The RNA helicase, eIF4A-1, is required for ovule development and cell size homeostasis in Arabidopsis

Awduron Sefydliadau
  • Maxwell S. Bush(Awdur)
    John Innes Centre
  • Natalie Crowe(Awdur)
    University of East Anglia
    John Innes Centre
  • Tao Zheng(Awdur)
    John Innes Centre
    Zhejiang Academy of Agricultural Science
  • John Doonan(Awdur)
    John Innes Centre
Math Erthygl
Iaith wreiddiolSaesneg
Tudalennau (o-i)989-1004
CyfnodolynPlant Journal
Cyfrol84
Rhif y cyfnodolyn5
Dyddiad ar-lein cynnar07 Rhag 2015
Dangosyddion eitem ddigidol (DOIs)
StatwsCyhoeddwyd - Rhag 2015
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eIF4A is a highly conserved RNA-stimulated ATPase and helicase involved in the initiation of mRNA translation. The Arabidopsis genome encodes 2 isoforms, one of which (eIF4A-1) is required for the coordination between cell cycle progression and cell size. A T-DNA mutant eif4a1 line, with reduced eIF4A protein levels, displays slow growth, reduced lateral root formation, delayed flowering and abnormal ovule development. Loss of eIF4A-1 reduces the proportion of mitotic cells in the root meristem and perturbs the relationship between cell size and cell cycle progression. Several cell cycle reporter proteins, particularly those expressed at G2/M, have reduced expression in eif4a1 mutant meristems. Single eif4a1 mutants are semi-sterile and show aberrant ovule growth, while double eif4a1/eif4a2 homozygous mutants could not be recovered, indicating that eIF4A function is essential for plant growth and development.

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