Temporal and spatial control of transgene expression using a heat-inducible promoter in transgenic wheat

Awduron Sefydliadau
  • Jacqueline Freeman(Awdur)
  • Caroline A. Sparks(Awdur)
    Rothamsted Research
  • Jevon West(Awdur)
    Rothamsted Research
  • Peter R Shewry(Awdur)
    Rothamsted Research
  • Huw Jones(Awdur)
Math Erthygl
Iaith wreiddiolSaesneg
Tudalennau (o-i)788-96
Nifer y tudalennau9
CyfnodolynPlant Biotechnology Journal
Cyfrol9
Rhif y cyfnodolyn7
Dangosyddion eitem ddigidol (DOIs)
StatwsCyhoeddwyd - Medi 2011
Cysylltiadau
Cysylltiad parhaol
Arddangos ystadegau lawrlwytho
Gweld graff cysylltiadau
Fformatau enwi

Crynodeb

Constitutive promoters are widely used to functionally characterise plant genes in transgenic plants, but their lack of specificity and poor control over protein expression can be a major disadvantage. On the other hand, promoters that provide precise regulation of temporal or spatial transgene expression facilitate such studies by targeting over-expression or knockdown of target genes to specific tissues and/or at particular developmental stages. Here, we used the uidA (beta-glucuronidase, GUS) reporter gene to demonstrate that the barley Hvhsp17 gene promoter can be induced by heat treatment of 38-40 °C for 1-2 h in transgenic wheat. The GUS enzyme was expressed only in those tissues directly exposed to heat and not in neighbouring leaf tissues. The induction of HSP::GUS was demonstrated in all organs and tissues tested, but expression in older tissues was lower. Generally, proximal root sections showed less GUS activity than in root tips. This heat-inducible promoter provides the ability to investigate the function of candidate genes by overexpression or by down-regulation of target gene expression (for example by RNAi) in selected tissues or developmental stages of a transgenic plant, limited only by the ability to apply a heat shock to the selected tissues. It also allows the investigation of genes that would be lethal or reduce fertility if expressed constitutively.

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