Fatty acid oxidation products (‘green odour’) released from perennial ryegrass following biotic and abiotic stress, potentially have antimicrobial properties against the rumen microbiota resulting in decreased biohydrogenation

Standard

Fatty acid oxidation products (‘green odour’) released from perennial ryegrass following biotic and abiotic stress, potentially have antimicrobial properties against the rumen microbiota resulting in decreased biohydrogenation. / Huws, S. A.; Scott, M. B.; Tweed, J. K. S.; Lee, M. R. F.

Yn: Journal of Applied Microbiology, Cyfrol 115, Rhif 5, 11.2013, t. 1081-1090.

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygl

Bibtex - Download

@article{968607dd39624ed5b786bc3deaa46a0c,
title = "Fatty acid oxidation products ({\textquoteleft}green odour{\textquoteright}) released from perennial ryegrass following biotic and abiotic stress, potentially have antimicrobial properties against the rumen microbiota resulting in decreased biohydrogenation",
abstract = "AimsIn this experiment, we investigated the effect of {\textquoteleft}green odour{\textquoteright} products typical of those released from fresh forage postabiotic and biotic stresses on the rumen microbiota and lipid metabolism.Methods and ResultsHydroperoxyoctadecatrienoic acid (HP), a combination of salicylic and jasmonic acid (T), and a combination of both (HPT) were incubated in vitro in the presence of freeze-dried ground silage and rumen fluid, under rumen-like conditions. 16S rRNA (16S cDNA) HaeIII-based terminal restriction fragment length polymorphism-based (T-RFLP) dendrograms, canonical analysis of principal coordinates graphs, peak number and Shanon-Weiner diversity indices show that HP, T and HPT likely had antimicrobial effects on the microbiota compared to control incubations. Following 6 h of in vitro incubation, 15·3% of 18:3n-3 and 4·4% of 18:2n-6 was biohydrogenated in control incubations, compared with 1·3, 9·4 and 8·3% of 18:3n-3 for HP, T and HPT treatments, respectively, with negligible 18:2n-6 biohydrogenation seen. T-RFLP peaks lost due to application of HP, T and HPT likely belonged to as yet uncultured bacteria within numerous genera.ConclusionsHydroperoxyoctadecatrienoic acid, T and HPT released due to plant stress potentially have an antimicrobial effect on the rumen microbiota, which may explain the decreased biohydrogenation in vitro.Significance and Impact of the StudyThese data suggest that these volatile chemicals may be responsible for the higher summer n-3 content of bovine milk.",
keywords = "bacteria, biohydrogenation, fatty acid, green odour, hydroperoxides, jasmonic acid, rumen, salicylic acid",
author = "Huws, {S. A.} and Scott, {M. B.} and Tweed, {J. K. S.} and Lee, {M. R. F.}",
year = "2013",
month = nov,
doi = "10.1111/jam.12314",
language = "English",
volume = "115",
pages = "1081--1090",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley",
number = "5",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Fatty acid oxidation products (‘green odour’) released from perennial ryegrass following biotic and abiotic stress, potentially have antimicrobial properties against the rumen microbiota resulting in decreased biohydrogenation

AU - Huws, S. A.

AU - Scott, M. B.

AU - Tweed, J. K. S.

AU - Lee, M. R. F.

PY - 2013/11

Y1 - 2013/11

N2 - AimsIn this experiment, we investigated the effect of ‘green odour’ products typical of those released from fresh forage postabiotic and biotic stresses on the rumen microbiota and lipid metabolism.Methods and ResultsHydroperoxyoctadecatrienoic acid (HP), a combination of salicylic and jasmonic acid (T), and a combination of both (HPT) were incubated in vitro in the presence of freeze-dried ground silage and rumen fluid, under rumen-like conditions. 16S rRNA (16S cDNA) HaeIII-based terminal restriction fragment length polymorphism-based (T-RFLP) dendrograms, canonical analysis of principal coordinates graphs, peak number and Shanon-Weiner diversity indices show that HP, T and HPT likely had antimicrobial effects on the microbiota compared to control incubations. Following 6 h of in vitro incubation, 15·3% of 18:3n-3 and 4·4% of 18:2n-6 was biohydrogenated in control incubations, compared with 1·3, 9·4 and 8·3% of 18:3n-3 for HP, T and HPT treatments, respectively, with negligible 18:2n-6 biohydrogenation seen. T-RFLP peaks lost due to application of HP, T and HPT likely belonged to as yet uncultured bacteria within numerous genera.ConclusionsHydroperoxyoctadecatrienoic acid, T and HPT released due to plant stress potentially have an antimicrobial effect on the rumen microbiota, which may explain the decreased biohydrogenation in vitro.Significance and Impact of the StudyThese data suggest that these volatile chemicals may be responsible for the higher summer n-3 content of bovine milk.

AB - AimsIn this experiment, we investigated the effect of ‘green odour’ products typical of those released from fresh forage postabiotic and biotic stresses on the rumen microbiota and lipid metabolism.Methods and ResultsHydroperoxyoctadecatrienoic acid (HP), a combination of salicylic and jasmonic acid (T), and a combination of both (HPT) were incubated in vitro in the presence of freeze-dried ground silage and rumen fluid, under rumen-like conditions. 16S rRNA (16S cDNA) HaeIII-based terminal restriction fragment length polymorphism-based (T-RFLP) dendrograms, canonical analysis of principal coordinates graphs, peak number and Shanon-Weiner diversity indices show that HP, T and HPT likely had antimicrobial effects on the microbiota compared to control incubations. Following 6 h of in vitro incubation, 15·3% of 18:3n-3 and 4·4% of 18:2n-6 was biohydrogenated in control incubations, compared with 1·3, 9·4 and 8·3% of 18:3n-3 for HP, T and HPT treatments, respectively, with negligible 18:2n-6 biohydrogenation seen. T-RFLP peaks lost due to application of HP, T and HPT likely belonged to as yet uncultured bacteria within numerous genera.ConclusionsHydroperoxyoctadecatrienoic acid, T and HPT released due to plant stress potentially have an antimicrobial effect on the rumen microbiota, which may explain the decreased biohydrogenation in vitro.Significance and Impact of the StudyThese data suggest that these volatile chemicals may be responsible for the higher summer n-3 content of bovine milk.

KW - bacteria

KW - biohydrogenation

KW - fatty acid

KW - green odour

KW - hydroperoxides

KW - jasmonic acid

KW - rumen

KW - salicylic acid

UR - http://hdl.handle.net/2160/12477

U2 - 10.1111/jam.12314

DO - 10.1111/jam.12314

M3 - Article

VL - 115

SP - 1081

EP - 1090

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 5

ER -

Gweld graff cysylltiadau
Fformatau enwi