Efficient method for rapid multiplication of clean and healthy willow clones via in vitro propagation with broad genotype applicability
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Efficient method for rapid multiplication of clean and healthy willow clones via in vitro propagation with broad genotype applicability. / Palomo-Rios, Elena; Macalpine, William; Shield, Ian et al.
Yn: Canadian Journal of Forest Research, Cyfrol 45, Rhif 11, 01.11.2015, t. 1662-1667.Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid
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TY - JOUR
T1 - Efficient method for rapid multiplication of clean and healthy willow clones via in vitro propagation with broad genotype applicability
AU - Palomo-Rios, Elena
AU - Macalpine, William
AU - Shield, Ian
AU - Amey, Joanna
AU - Karaoglu, Cuma
AU - West, Jevon
AU - Hanley, Steven
AU - Krygier, Richard
AU - Karp, Angela
AU - Jones, Huw D
PY - 2015/11/1
Y1 - 2015/11/1
N2 - Willow is a versatile crop with considerable potential as a source of renewable biomass for bioenergy. Although breeding new varieties takes less time compared with some other tree species, producing new willow varieties is still a slow, labour-intensive process, partly because clonally propagating the results of each cross is a bottleneck early in the breeding scheme. In this paper, we describe a facile, rapid method for the in vitro culture of a wide range of willow genotypes. We have developed a combination of media and methods for efficient tissue-culture propagation to rapidly multiply individual plants and simultaneously produce clean, stock germplasm applicable to a wide range of willow genotypes that can be phytosanitary tested to demonstrate their disease-free status. The micropropagation method described could generate in the order of 5000 viable, transplantable clones from a single plant in just 24 weeks and was used to produce phytosanitary tested breeding material for export to overcome restriction on the international transport of woody cuttings. This method could represent a valuable biotechnology adjunct to willow breeding programmes and could accommodate early selection via molecular or biochemical markers.
AB - Willow is a versatile crop with considerable potential as a source of renewable biomass for bioenergy. Although breeding new varieties takes less time compared with some other tree species, producing new willow varieties is still a slow, labour-intensive process, partly because clonally propagating the results of each cross is a bottleneck early in the breeding scheme. In this paper, we describe a facile, rapid method for the in vitro culture of a wide range of willow genotypes. We have developed a combination of media and methods for efficient tissue-culture propagation to rapidly multiply individual plants and simultaneously produce clean, stock germplasm applicable to a wide range of willow genotypes that can be phytosanitary tested to demonstrate their disease-free status. The micropropagation method described could generate in the order of 5000 viable, transplantable clones from a single plant in just 24 weeks and was used to produce phytosanitary tested breeding material for export to overcome restriction on the international transport of woody cuttings. This method could represent a valuable biotechnology adjunct to willow breeding programmes and could accommodate early selection via molecular or biochemical markers.
KW - willow breeding (Salix)
KW - in vitro culture
KW - multiplication
KW - propagation
KW - phytosanitary
KW - SALIX
KW - MICROPROPAGATION
KW - IMPROVEMENT
KW - BIOENERGY
KW - PLANTS
KW - MEDIA
UR - http://hdl.handle.net/2160/43027
U2 - 10.1139/cjfr-2015-0055
DO - 10.1139/cjfr-2015-0055
M3 - Article
VL - 45
SP - 1662
EP - 1667
JO - Canadian Journal of Forest Research
JF - Canadian Journal of Forest Research
SN - 0045-5067
IS - 11
ER -