Efficient method for rapid multiplication of clean and healthy willow clones via in vitro propagation with broad genotype applicability

Awduron Sefydliadau
  • Elena Palomo-Rios(Awdur)
    Rothamsted Research
  • William Macalpine(Awdur)
    Rothamsted Research
  • Ian Shield(Awdur)
    Rothamsted Research
  • Joanna Amey(Awdur)
    Rothamsted Research
  • Cuma Karaoglu(Awdur)
    Central Research Institute for Field Crops
  • Jevon West(Awdur)
    Rothamsted Research
  • Steven Hanley(Awdur)
    Rothamsted Research
  • Richard Krygier(Awdur)
    Canadian Forest Service
  • Angela Karp(Awdur)
    Rothamsted Research
  • Huw Jones(Awdur)
    Rothamsted Research
Math Erthygl
Iaith wreiddiolSaesneg
Tudalennau (o-i)1662-1667
Nifer y tudalennau6
CyfnodolynCanadian Journal of Forest Research
Rhif y cyfnodolyn11
Dangosyddion eitem ddigidol (DOIs)
StatwsCyhoeddwyd - 01 Tach 2015
Cyhoeddwyd yn allanolIe
Cysylltiad parhaol
Gweld graff cysylltiadau
Fformatau enwi


Willow is a versatile crop with considerable potential as a source of renewable biomass for bioenergy. Although breeding new varieties takes less time compared with some other tree species, producing new willow varieties is still a slow, labour-intensive process, partly because clonally propagating the results of each cross is a bottleneck early in the breeding scheme. In this paper, we describe a facile, rapid method for the in vitro culture of a wide range of willow genotypes. We have developed a combination of media and methods for efficient tissue-culture propagation to rapidly multiply individual plants and simultaneously produce clean, stock germplasm applicable to a wide range of willow genotypes that can be phytosanitary tested to demonstrate their disease-free status. The micropropagation method described could generate in the order of 5000 viable, transplantable clones from a single plant in just 24 weeks and was used to produce phytosanitary tested breeding material for export to overcome restriction on the international transport of woody cuttings. This method could represent a valuable biotechnology adjunct to willow breeding programmes and could accommodate early selection via molecular or biochemical markers.