Dynamics of initial colonisation of non-conserved perennial ryegrass by anaerobic fungi in the bovine rumen

TY - JOUR

T1 - Dynamics of initial colonisation of non-conserved perennial ryegrass by anaerobic fungi in the bovine rumen

AU - Edwards, Joan Elizabeth

AU - Kingston-Smith, A. H.

AU - Jimenez, Hugo R.

AU - Huws, S. A.

AU - Skot, K. P.

AU - Griffith, G. W.

AU - McEwan, N. R.

AU - Theodorou, M. K.

N1 - Edwards, J. E., Kingston-Smith, A. H., Jimenez, H. R., Huws, S. A., Skøt, K. P., Griffith, G. W., McEwan, N. R., Theodorou, M. K. (2008). Dynamics of initial colonisation of non-conserved perennial ryegrass by anaerobic fungi in the bovine rumen. FEMS Microbiology Ecology, 66, (3), pp. 537-545 KEYWORDS: Neocallimastigales • rumen • colonization • ITS1 • 5.8S rRNA gene ABSTRACT IMPF: 03.33 RONO: DB 03135 Funder: BBSRC RONO: BBS/E/W/00003135A

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Anaerobic fungi (Neocallimastigales) are active degraders of fibrous plant material in the rumen. However, only limited information is available relating to how quickly they colonize ingested feed particles. The aim of this study was to determine the dynamics of initial colonization of forage by anaerobic fungi in the rumen and the impact of different postsampling wash procedures used to remove loosely associated microorganisms. Neocallimastigales-specific molecular techniques were optimized to ensure maximal coverage before application to assess the population size (quantitative PCR) and composition (automated ribosomal intergenic spacer analysis) of the colonizing anaerobic fungi. Colonization of perennial ryegrass (PRG) was evident within 5 min, with no consistent effect of time or wash procedure on fungal population composition. Wash procedure had no effect on population size unlike time, which had a significant effect. Colonizing fungal population size continued to increase over the incubation period after an initial lag of c. 4 min. This dynamic differs from that reported previously for rumen bacteria, where substantial colonization of PRG occurred within 5 min. The observed delay in colonization of plant material by anaerobic fungi is suggested to be primarily mediated by the time taken for fungal zoospores to locate, attach and encyst on plant material.

AB - Anaerobic fungi (Neocallimastigales) are active degraders of fibrous plant material in the rumen. However, only limited information is available relating to how quickly they colonize ingested feed particles. The aim of this study was to determine the dynamics of initial colonization of forage by anaerobic fungi in the rumen and the impact of different postsampling wash procedures used to remove loosely associated microorganisms. Neocallimastigales-specific molecular techniques were optimized to ensure maximal coverage before application to assess the population size (quantitative PCR) and composition (automated ribosomal intergenic spacer analysis) of the colonizing anaerobic fungi. Colonization of perennial ryegrass (PRG) was evident within 5 min, with no consistent effect of time or wash procedure on fungal population composition. Wash procedure had no effect on population size unlike time, which had a significant effect. Colonizing fungal population size continued to increase over the incubation period after an initial lag of c. 4 min. This dynamic differs from that reported previously for rumen bacteria, where substantial colonization of PRG occurred within 5 min. The observed delay in colonization of plant material by anaerobic fungi is suggested to be primarily mediated by the time taken for fungal zoospores to locate, attach and encyst on plant material.

KW - Neocallimastigales

KW - rumen

KW - colonization

KW - ITS1

KW - 5.8S rRNA gene

U2 - 10.1111/j.1574-6941.2008.00563.x

DO - 10.1111/j.1574-6941.2008.00563.x

M3 - Article

VL - 66

SP - 537

EP - 545

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 3

ER -