Arabidopsis T-DNA insertional lines for CDC25 are hypersensitive to hydroxyurea but not to zeocin or salt stress

Awduron Sefydliadau
  • Natasha D Spadafora(Awdur)
    Prifysgol Caerdydd | Cardiff University
  • John Doonan(Awdur)
  • Robert J Herbert(Awdur)
    University of Worcester
  • M Beatrice Bitonti(Awdur)
    University of Calabria
  • Emily Wallace(Awdur)
    Prifysgol Caerdydd | Cardiff University
  • Hilary J Rogers(Awdur)
    Prifysgol Caerdydd | Cardiff University
  • Dennis Francis(Awdur)
    Prifysgol Caerdydd | Cardiff University
Math Erthygl
Iaith wreiddiolSaesneg
Tudalennau (o-i)1183-1192
Nifer y tudalennau10
CyfnodolynAnnals of Botany
Rhif y cyfnodolyn7
Dyddiad ar-lein cynnar20 Gorff 2010
Dangosyddion eitem ddigidol (DOIs)
StatwsCyhoeddwyd - 07 Mai 2011
Gweld graff cysylltiadau
Fformatau enwi


BACKGROUND AND AIMS: In yeasts and animals, cyclin-dependent kinases are key regulators of cell cycle progression and are negatively and positively regulated by WEE1 kinase and CDC25 phosphatase, respectively. In higher plants a full-length orthologue of CDC25 has not been isolated but a shorter gene with homology only to the C-terminal catalytic domain is present. The Arabidopis thaliana;CDC25 can act as a phosphatase in vitro. Since in arabidopsis, WEE1 plays an important role in the DNA damage/DNA replication checkpoints, the role of Arath;CDC25 in conditions that induce these checkpoints or induce abiotic stress was tested. Methods arath;cdc25 T-DNA insertion lines, Arath;CDC25 over-expressing lines and wild type were challenged with hydroxyurea (HU) and zeocin, substances that stall DNA replication and damage DNA, respectively, together with an abiotic stressor, NaCl. A molecular and phenotypic assessment was made of all genotypes Key

RESULTS: There was a null phenotypic response to perturbation of Arath;CDC25 expression under control conditions. However, compared with wild type, the arath;cdc25 T-DNA insertion lines were hypersensitive to HU, whereas the Arath;CDC25 over-expressing lines were relatively insensitive. In particular, the over-expressing lines consistently outgrew the T-DNA insertion lines and wild type when challenged with HU. All genotypes were equally sensitive to zeocin and NaCl.

CONCLUSIONS: Arath;CDC25 plays a role in overcoming stress imposed by HU, an agent know to induce the DNA replication checkpoint in arabidopsis. However, it could not enhance tolerance to either a zeocin treatment, known to induce DNA damage, or salinity stress.