A general model of error-prone PCR

Awduron Sefydliadau
Math Erthygl
Iaith wreiddiolSaesneg
Tudalennau (o-i)497-509
Nifer y tudalennau13
CyfnodolynJournal of Theoretical Biology
Rhif y cyfnodolyn4
Dyddiad ar-lein cynnar29 Ion 2005
Dangosyddion eitem ddigidol (DOIs)
StatwsCyhoeddwyd - 21 Meh 2005
Gweld graff cysylltiadau
Fformatau enwi


In this paper, we generalise a previously-described model of the error-prone polymerase chain reaction (PCR) reaction to conditions of arbitrarily variable amplification efficiency and initial population size. Generalisation of the model to these conditions improves the correspondence to observed and expected behaviours of PCR, and restricts the extent to which the model may explore sequence space for a prescribed set of parameters. Error-prone PCR in realistic reaction conditions is predicted to be less effective at generating grossly divergent sequences than the original model. The estimate of mutation rate per cycle by sampling sequences from an in vitro PCR experiment is correspondingly affected by the choice of model and parameters.